Validation of glutathione quantitation from STEAM spectra against edited 1H NMR spectroscopy at 4T: application to schizophrenia.

Details

Serval ID
serval:BIB_8F28D1C3F427
Type
Article: article from journal or magazin.
Collection
Publications
Title
Validation of glutathione quantitation from STEAM spectra against edited 1H NMR spectroscopy at 4T: application to schizophrenia.
Journal
Magma
Author(s)
Terpstra M., Vaughan T.J., Ugurbil K., Lim K.O., Schulz S.C., Gruetter R.
ISSN
0968-5243 (Print)
ISSN-L
0968-5243
Publication state
Published
Issued date
2005
Volume
18
Number
5
Pages
276-282
Language
english
Notes
Publication types: Controlled Clinical Trial ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Validation StudiesPublication Status: ppublish
Abstract
OBJECTIVE: Quantitation of glutathione (GSH) in the human brain in vivo using short echo time 1H NMR spectroscopy is challenging because GSH resonances are not easily resolved. The main objective of this study was to validate such quantitation in a clinically relevant population using the resolved GSH resonances provided by edited spectroscopy. A secondary objective was to compare several of the neurochemical concentrations quantified along with GSH using LCModel analysis of short echo time spectra in schizophrenia versus control.
MATERIALS AND METHODS: GSH was quantified at 4T from short echo STEAM spectra and MEGA-PRESS edited spectra from identical volumes of interest (anterior cingulate) in ten volunteers. Neurochemical profiles were quantified in nine controls and 13 medicated schizophrenic patients.
RESULTS: GSH concentrations as quantified using STEAM, 1.6 +/- 0.4 micromol/g (mean +/- SD, n = 10), were within error of those quantified using edited spectra, 1.4 +/- 0.4 micromol/g, and were not different (p = 0.4). None of the neurochemical measurements reached sufficient statistical power to detect differences smaller than 10% in schizophrenia versus control. As such, no differences were observed.
CONCLUSIONS: Human brain GSH concentrations can be quantified in a clinical setting using short-echo time STEAM spectra at 4T.
Keywords
Adult, Algorithms, Biological Markers/analysis, Female, Glutathione/analysis, Humans, Magnetic Resonance Spectroscopy/methods, Male, Protons/diagnostic use, Reproducibility of Results, Schizophrenia/diagnosis, Schizophrenia/metabolism, Sensitivity and Specificity, Single-Blind Method
Pubmed
Web of science
Create date
04/08/2010 16:28
Last modification date
03/03/2018 19:20
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