1H-[13C] NMR spectroscopy of the rat brain during infusion of [2-13C] acetate at 14.1 T.

Details

Serval ID
serval:BIB_8ED92F005E43
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
1H-[13C] NMR spectroscopy of the rat brain during infusion of [2-13C] acetate at 14.1 T.
Journal
Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine
Author(s)
Xin L., Mlynárik V., Lanz B., Frenkel H., Gruetter R.
ISSN
1522-2594 (Electronic)
ISSN-L
0740-3194
Publication state
Published
Issued date
08/2010
Volume
64
Number
2
Pages
334-340
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Full signal intensity (1)H-[(13)C] NMR spectroscopy, combining a preceding (13)C-editing block based on an inversion BISEP (B(1)-insensitive spectral editing pulse) with a spin-echo coherence-based localization, was developed and implemented at 14.1 T. (13)C editing of the proposed scheme was achieved by turning on and off the (13)C adiabatic full passage in the (13)C-editing block to prepare inverted and noninverted (13)C-coupled (1)H coherences along the longitudinal axis prior to localization. The novel (1)H-[(13)C] NMR approach was applied in vivo under infusion of the glia-specific substrate [2-(13)C] acetate. Besides a approximately 50% improvement in sensitivity, spectral dispersion was enhanced at 14.1 T, especially for J-coupled metabolites such as glutamate and glutamine. A more distinct spectral structure at 1.9-2.2 ppm(parts per million) was observed, e.g., glutamate C3 showed a doublet pattern in both simulated (1)H spectrum and in vivo (13)C-edited (1)H NMR spectra. Besides (13)C time courses of glutamate C4 and glutamine C4, the time courses of glutamate C3 and glutamine C3 obtained by (1)H-[(13)C] NMR spectroscopy were reported for the first time. Such capability should greatly improve the ability to study neuron-glial metabolism using (1)H-observed (13)C-edited NMR spectroscopy.
Keywords
Acetates/administration & dosage, Acetates/pharmacokinetics, Animals, Brain/metabolism, Carbon Isotopes/administration & dosage, Carbon Isotopes/pharmacokinetics, Feasibility Studies, Isotope Labeling/methods, Magnetic Resonance Spectroscopy/methods, Protons, Radiopharmaceuticals/administration & dosage, Radiopharmaceuticals/pharmacokinetics, Rats, Rats, Sprague-Dawley
Pubmed
Web of science
Create date
04/08/2010 15:28
Last modification date
20/08/2019 14:52
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