Chromosome segregation drives division site selection in Streptococcus pneumoniae.

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State: Public
Version: author
Serval ID
serval:BIB_8EBF2BBCBA37
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Chromosome segregation drives division site selection in Streptococcus pneumoniae.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
van Raaphorst R., Kjos M., Veening J.W.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
18/07/2017
Peer-reviewed
Oui
Volume
114
Number
29
Pages
E5959-E5968
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Accurate spatial and temporal positioning of the tubulin-like protein FtsZ is key for proper bacterial cell division. <i>Streptococcus pneumoniae</i> (pneumococcus) is an oval-shaped, symmetrically dividing opportunistic human pathogen lacking the canonical systems for division site control (nucleoid occlusion and the Min-system). Recently, the early division protein MapZ was identified and implicated in pneumococcal division site selection. We show that MapZ is important for proper division plane selection; thus, the question remains as to what drives pneumococcal division site selection. By mapping the cell cycle in detail, we show that directly after replication both chromosomal origin regions localize to the future cell division sites, before FtsZ. Interestingly, Z-ring formation occurs coincidently with initiation of DNA replication. Perturbing the longitudinal chromosomal organization by mutating the condensin SMC, by CRISPR/Cas9-mediated chromosome cutting, or by poisoning DNA decatenation resulted in mistiming of MapZ and FtsZ positioning and subsequent cell elongation. Together, we demonstrate an intimate relationship between DNA replication, chromosome segregation, and division site selection in the pneumococcus, providing a simple way to ensure equally sized daughter cells.
Keywords
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, CRISPR-Cas Systems, Cell Division, Chromosome Segregation, Cytoskeletal Proteins/genetics, Cytoskeletal Proteins/metabolism, DNA Polymerase III/genetics, DNA Polymerase III/metabolism, DNA Replication, Green Fluorescent Proteins/genetics, Green Fluorescent Proteins/metabolism, Origin Recognition Complex, Streptococcus pneumoniae/cytology, Streptococcus pneumoniae/genetics, FtsZ, SMC, Streptococcus pneumoniae, cell division, chromosome organization
Pubmed
Web of science
Open Access
Yes
Create date
27/07/2017 14:05
Last modification date
20/08/2019 15:52
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