The protein Mago provides a link between splicing and mRNA localization.
Details
Serval ID
serval:BIB_8E6DB955A1BC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The protein Mago provides a link between splicing and mRNA localization.
Journal
EMBO Reports
ISSN
1469-221X (Print)
ISSN-L
1469-221X
Publication state
Published
Issued date
2001
Volume
2
Number
12
Pages
1119-1124
Language
english
Abstract
The proteins Mago and Y14 are evolutionarily conserved binding partners. Y14 is a component of the exon-exon junction complex (EJC), deposited by the spliceosome upstream of messenger RNA (mRNA) exon-exon junctions. The EJC is implicated in post-splicing events such as mRNA nuclear export and nonsense-mediated mRNA decay. Drosophila Mago is essential for the localization of oskar mRNA to the posterior pole of the oocyte, but the functional role of Mago in other species is unknown. We show that Mago is a bona fide component of the EJC. Like Y14, Mago escorts spliced mRNAs to the cytoplasm, providing a direct functional link between splicing and the downstream process of mRNA localization. Mago/Y14 heterodimers are essential in cultured Drosophila cells. Taken together, these results suggest that, in addition to its specialized function in mRNA localization, Mago plays an essential role in other steps of mRNA metabolism.
Keywords
Animals, Biological Transport, Cell Division, Cell Line, Cell Nucleus Structures/metabolism, Cytoplasm/metabolism, DNA/genetics, Dimerization, Drosophila/cytology, Drosophila/genetics, Drosophila Proteins, Evolution, Molecular, Exons/genetics, HeLa Cells, Humans, Male, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Polymerase Chain Reaction, Protein Binding, RNA Splicing/genetics, RNA, Messenger/chemistry, RNA, Messenger/genetics, RNA-Binding Proteins/genetics, RNA-Binding Proteins/metabolism, Testis, Transfection, Xenopus
Pubmed
Web of science
Open Access
Yes
Create date
12/12/2012 12:22
Last modification date
20/08/2019 15:52
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