Diabetes imaging-quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy.

Details

Serval ID
serval:BIB_8E625B139290
Type
Article: article from journal or magazin.
Collection
Publications
Title
Diabetes imaging-quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy.
Journal
Biomedical optics express
Author(s)
Berclaz C., Goulley J., Villiger M., Pache C., Bouwens A., Martin-Williams E., Van de Ville D., Davison A.C., Grapin-Botton A., Lasser T.
ISSN
2156-7085 (Electronic)
ISSN-L
2156-7085
Publication state
Published
Issued date
01/06/2012
Peer-reviewed
Oui
Volume
3
Number
6
Pages
1365-1380
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Diabetes is characterized by hyperglycemia that can result from the loss of pancreatic insulin secreting β-cells in the islets of Langerhans. We analyzed ex vivo the entire gastric and duodenal lobes of a murine pancreas using extended-focus Optical Coherence Microscopy (xfOCM). To identify and quantify the islets of Langerhans observed in xfOCM tomograms we implemented an active contour algorithm based on the level set method. We show that xfOCM reveals a three-dimensional islet distribution consistent with Optical Projection Tomography, albeit with a higher resolution that also enables the detection of the smallest islets (≤ 8000 μm(3)). Although this category of the smallest islets represents only a negligible volume compared to the total β-cell volume, a recent study suggests that these islets, located at the periphery, are the first to be destroyed when type I diabetes develops. Our results underline the capability of xfOCM to contribute to the understanding of the development of diabetes, especially when considering islet volume distribution instead of the total β-cell volume only.
Keywords
(100.6890) Three-dimensional image processing, (170.0170) Medical optics and biotechnology, (170.1420) Biology, (170.4500) Optical coherence tomography, (170.6935) Tissue characterization
Pubmed
Web of science
Open Access
Yes
Create date
22/10/2014 8:02
Last modification date
01/03/2024 16:40
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