Regulation of enzyme synthesis in the arginine deiminase pathway of Pseudomonas aeruginosa.

Details

Serval ID
serval:BIB_8E54328692C7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Regulation of enzyme synthesis in the arginine deiminase pathway of Pseudomonas aeruginosa.
Journal
Journal of Bacteriology
Author(s)
Mercenier A., Simon J.P., Vander Wauven C., Haas D., Stalon V.
ISSN
0021-9193 (Print)
ISSN-L
0021-9193
Publication state
Published
Issued date
1980
Volume
144
Number
1
Pages
159-163
Language
english
Abstract
The three enzymes of the arginine deiminase pathway in Pseudomonas aeruginosa strain PAO were induced strongly (50- to 100-fold) by a shift from aerobic growth conditions to very low oxygen tension. Arginine in the culture medium was not essential for induction, but increased the maximum enzyme levels twofold. The induction of the three enzymes arginine deiminase (EC 3.5.3.6), catabolic ornithine carbamoyltransferase (EC 2.1.3.3), and carbamate kinase (EC 2.7.2.3) appeared to be coordinate. Catabolic ornithine carbamoyltransferase was studied in most detail. Nitrate and nitrite, which can replace oxygen as terminal electron acceptors in P. aeruginosa, partially prevented enzyme induction by low oxygen tension in the wild-type strain, but not in nar (nitrate reductase-negative) mutants. Glucose was found to exert catabolite repression of the deiminase pathway. Generally, conditions of stress, such as depletion of the carbon and energy source or the phosphate source, resulted in induced synthesis of catabolic ornithine carbamoyltransferase. The induction of the deiminase pathway is thought to mobilize intra- and extracellular reserves of arginine, which is used as a source of adenosine 5'-triphosphate in the absence of respiration.
Keywords
Arginine/pharmacology, Carbamates/biosynthesis, Enzyme Induction/drug effects, Hydrolases/biosynthesis, Nitrates/pharmacology, Nitrites/pharmacology, Ornithine Carbamoyltransferase/biosynthesis, Oxygen/physiology, Phosphotransferases/biosynthesis, Phosphotransferases (Carboxyl Group Acceptor), Pseudomonas aeruginosa/enzymology
Pubmed
Web of science
Create date
25/01/2008 17:01
Last modification date
20/08/2019 14:52
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