Level of accumulation of epoxy fatty acid in Arabidopsis thaliana expressing a linoleic acid delta12-epoxygenase is influenced by the availability of the substrate linoleic acid.

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Serval ID
serval:BIB_8E4A6021D098
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Level of accumulation of epoxy fatty acid in Arabidopsis thaliana expressing a linoleic acid delta12-epoxygenase is influenced by the availability of the substrate linoleic acid.
Journal
Theoretical and Applied Genetics
Author(s)
Rezzonico E., Moire L., Delessert S., Poirier Y.
ISSN
0040-5752 (Print)
ISSN-L
0040-5752
Publication state
Published
Issued date
2004
Volume
109
Number
5
Pages
1077-1082
Language
english
Abstract
Arabidopsis thaliana (L.) Heynh. expressing the Crepis palaestina (L.) linoleic acid delta12-epoxygenase in its developing seeds typically accumulates low levels of vernolic acid (12,13-epoxy-octadec-cis-9-enoic acid) in comparison to levels found in seeds of the native C. palaestina. In order to determine some of the factors limiting the accumulation of this unusual fatty acid, we have examined the effects of increasing the availability of linoleic acid (9cis, 12cis-octadecadienoic acid), the substrate of the delta12-epoxygenase, on the quantity of epoxy fatty acids accumulating in transgenic A. thaliana. The addition of linoleic acid to liquid cultures of transgenic plants expressing the delta12-epoxygenase under the control of the cauliflower mosaic virus 35S promoter increased the amount of vernolic acid in vegetative tissues by 2.8-fold. In contrast, the addition to these cultures of linoelaidic acid (9trans, 12trans-octadecadienoic acid), which is not a substrate of the delta12-epoxygenase, resulted in a slight decrease in vernolic acid accumulation. Expression of the delta12-epoxygenase under the control of the napin promoter in the A. thaliana triple mutant fad3/fad7-1/fad9, which is deficient in the synthesis of tri-unsaturated fatty acids and has a 60% higher level of linoleic acid than the wild type, was found to increase the average vernolic acid content of the seeds by 55% compared to the expression of the delta12-epoxygenase in a wild-type background. Together, these results reveal that the availability of linoleic acid is an important factor affecting the synthesis of epoxy fatty acid in transgenic plants.
Keywords
Arabidopsis/genetics, Arabidopsis/metabolism, Caulimovirus/genetics, Epoxy Compounds, Gene Transfer Techniques, Linoleic Acid/metabolism, Oleic Acids/biosynthesis, Oxidoreductases/genetics, Oxidoreductases/metabolism, Plant Proteins/genetics, Plant Proteins/metabolism, Plants, Genetically Modified/genetics, Plants, Genetically Modified/metabolism, Promoter Regions, Genetic/genetics, Seeds/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 20:42
Last modification date
01/10/2019 7:18
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