On the evolution of chaperones and cochaperones and the expansion of proteomes across the Tree of Life.

Details

Serval ID
serval:BIB_8E0889F56271
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
On the evolution of chaperones and cochaperones and the expansion of proteomes across the Tree of Life.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Rebeaud M.E., Mallik S., Goloubinoff P., Tawfik D.S.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
25/05/2021
Peer-reviewed
Oui
Volume
118
Number
21
Pages
e2020885118
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Across the Tree of Life (ToL), the complexity of proteomes varies widely. Our systematic analysis depicts that from the simplest archaea to mammals, the total number of proteins per proteome expanded ∼200-fold. Individual proteins also became larger, and multidomain proteins expanded ∼50-fold. Apart from duplication and divergence of existing proteins, completely new proteins were born. Along the ToL, the number of different folds expanded ∼5-fold and fold combinations ∼20-fold. Proteins prone to misfolding and aggregation, such as repeat and beta-rich proteins, proliferated ∼600-fold and, accordingly, proteins predicted as aggregation-prone became 6-fold more frequent in mammalian compared with bacterial proteomes. To control the quality of these expanding proteomes, core chaperones, ranging from heat shock proteins 20 (HSP20s) that prevent aggregation to HSP60, HSP70, HSP90, and HSP100 acting as adenosine triphosphate (ATP)-fueled unfolding and refolding machines, also evolved. However, these core chaperones were already available in prokaryotes, and they comprise ∼0.3% of all genes from archaea to mammals. This challenge-roughly the same number of core chaperones supporting a massive expansion of proteomes-was met by 1) elevation of messenger RNA (mRNA) and protein abundances of the ancient generalist core chaperones in the cell, and 2) continuous emergence of new substrate-binding and nucleotide-exchange factor cochaperones that function cooperatively with core chaperones as a network.
Keywords
Tree of Life, chaperone network, cochaperones, core chaperones, expansion of proteomes
Pubmed
Web of science
Open Access
Yes
Create date
25/05/2021 9:48
Last modification date
26/06/2021 7:13
Usage data