Hydrogen sulfide measurement by headspace-gas chromatography-mass spectrometry (HS-GC-MS): application to gaseous samples and gas dissolved in muscle.

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Serval ID
serval:BIB_8DFE01387938
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Hydrogen sulfide measurement by headspace-gas chromatography-mass spectrometry (HS-GC-MS): application to gaseous samples and gas dissolved in muscle.
Journal
Journal of Analytical Toxicology
Author(s)
Varlet V., Giuliani N., Palmiere C., Maujean G., Augsburger M.
ISSN
1945-2403 (Electronic)
ISSN-L
0146-4760
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
39
Number
1
Pages
52-57
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
The aim of our study was to present a new headspace-gas chromatography-mass spectrometry (HS-GC-MS) method applicable to the routine determination of hydrogen sulfide (H(2)S) concentrations in biological and gaseous samples. The primary analytical drawback of the GC/MS methods for H(2)S measurement discussed in the literature was the absence of a specific H(2)S internal standard required to perform quantification. Although a deuterated hydrogen sulfide (D(2)S) standard is currently available, this standard is not often used because this standard is expensive and is only available in the gas phase. As an alternative approach, D(2)S can be generated in situ by reacting deuterated chloride with sodium sulfide; however, this technique can lead to low recovery yield and potential isotopic fractionation. Therefore, N(2)O was chosen for use as an internal standard. This method allows precise measurements of H(2)S concentrations in biological and gaseous samples. Therefore, a full validation using accuracy profile based on the β-expectation tolerance interval is presented. Finally, this method was applied to quantify H(2)S in an actual case of H(2)S fatal intoxication.
Pubmed
Web of science
Open Access
Yes
Create date
24/10/2014 13:46
Last modification date
25/09/2019 6:09
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