Label-free identification of activated T lymphocytes through tridimensional microsensors on chip.

Details

Serval ID
serval:BIB_8DDCB6B9A58E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Label-free identification of activated T lymphocytes through tridimensional microsensors on chip.
Journal
Biosensors & bioelectronics
Author(s)
Rollo E., Tenaglia E., Genolet R., Bianchi E., Harari A., Coukos G., Guiducci C.
ISSN
1873-4235 (Electronic)
ISSN-L
0956-5663
Publication state
Published
Issued date
15/08/2017
Peer-reviewed
Oui
Volume
94
Pages
193-199
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Label-free approaches to assess cell properties ideally suit the requirements of cell-based therapeutics, since they permit to characterize cells with minimal perturbation and manipulation, at the benefit of sample recovery and re-employment for treatment. For this reason, label-free techniques would find sensible application in adoptive T cell-based immunotherapy. In this work, we describe the label-free and single-cell detection of in vitro activated T lymphocytes in flow through an electrical impedance-based setup. We describe a novel platform featuring 3D free-standing microelectrodes presenting passive upstream and downstream extensions and integrated into microfluidic channels. We employ such device to measure the impedance change associated with T cell activation at electrical frequencies maximizing the difference between non-activated and activated T cells. Finally, we harness the impedance signature of unstimulated T cells to set a boundary separating activated and non-activated clones, so to characterize the selectivity and specificity of the system. In conclusion, the strategy here proposed highlights the possible employment of impedance to assess T cell activation in label-free.

Keywords
Biosensing Techniques/methods, Electric Impedance, Humans, Lymphocyte Activation/immunology, Microfluidic Analytical Techniques/methods, Single-Cell Analysis/methods, T-Lymphocytes/chemistry, T-Lymphocytes/immunology, 3D electrodes, Electrochemical impedance, Immunotherapy, Lab-on-chip devices, Microelectrodes, Microfluidic flow cytometry, Microfluidics, T cells
Pubmed
Web of science
Create date
21/03/2017 19:37
Last modification date
20/08/2019 15:51
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