PKA antagonizes CLASP-dependent microtubule stabilization to re-localize Pom1 and buffer cell size upon glucose limitation.

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State: Public
Version: Final published version
Serval ID
serval:BIB_8D3C480D64EF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
PKA antagonizes CLASP-dependent microtubule stabilization to re-localize Pom1 and buffer cell size upon glucose limitation.
Journal
Nature Communications
Author(s)
Kelkar M., Martin S.G.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
2015
Volume
6
Pages
8445
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
Publication Status: epublish
Abstract
Cells couple growth with division and regulate size in response to nutrient availability. In rod-shaped fission yeast, cell-size control occurs at mitotic commitment. An important regulator is the DYRK-family kinase Pom1, which forms gradients from cell poles and inhibits the mitotic activator Cdr2, itself localized at the medial cortex. Where and when Pom1 modulates Cdr2 activity is unclear as Pom1 medial cortical levels remain constant during cell elongation. Here we show that Pom1 re-localizes to cell sides upon environmental glucose limitation, where it strongly delays mitosis. This re-localization is caused by severe microtubule destabilization upon glucose starvation, with microtubules undergoing catastrophe and depositing the Pom1 gradient nucleator Tea4 at cell sides. Microtubule destabilization requires PKA/Pka1 activity, which negatively regulates the microtubule rescue factor CLASP/Cls1/Peg1, reducing CLASP's ability to stabilize microtubules. Thus, PKA signalling tunes CLASP's activity to promote Pom1 cell side localization and buffer cell size upon glucose starvation.
Keywords
Blotting, Western, Cell Polarity, Cell Size, Cyclic AMP-Dependent Protein Kinases/metabolism, Glucose/metabolism, Microscopy, Confocal, Microscopy, Fluorescence, Microtubule-Associated Proteins/metabolism, Microtubules/metabolism, Mitosis, Nuclear Proteins/metabolism, Protein Kinases/metabolism, Protein-Serine-Threonine Kinases/metabolism, Schizosaccharomyces/cytology, Schizosaccharomyces/metabolism, Schizosaccharomyces pombe Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
10/12/2015 22:54
Last modification date
20/08/2019 14:51
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