Inhibition of REV3 expression induces persistent DNA damage and growth arrest in cancer cells.

Details

Serval ID
serval:BIB_8D2BD2846110
Type
Article: article from journal or magazin.
Collection
Publications
Title
Inhibition of REV3 expression induces persistent DNA damage and growth arrest in cancer cells.
Journal
Neoplasia
Author(s)
Knobel P.A., Kotov I.N., Felley-Bosco E., Stahel R.A., Marti T.M.
ISSN
1476-5586 (Electronic)
ISSN-L
1476-5586
Publication state
Published
Issued date
2011
Volume
13
Number
10
Pages
961-970
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
REV3 is the catalytic subunit of DNA translesion synthesis polymerase ζ. Inhibition of REV3 expression increases the sensitivity of human cells to a variety of DNA-damaging agents and reduces the formation of resistant cells. Surprisingly, we found that short hairpin RNA-mediated depletion of REV3 per se suppresses colony formation of lung (A549, Calu-3), breast (MCF-7, MDA-MB-231), mesothelioma (IL45 and ZL55), and colon (HCT116 +/-p53) tumor cell lines, whereas control cell lines (AD293, LP9-hTERT) and the normal mesothelial primary culture (SDM104) are less affected. Inhibition of REV3 expression in cancer cells leads to an accumulation of persistent DNA damage as indicated by an increase in phospho-ATM, 53BP1, and phospho-H2AX foci formation, subsequently leading to the activation of the ATM-dependent DNA damage response cascade. REV3 depletion in p53-proficient cancer cell lines results in a G(1) arrest and induction of senescence as indicated by the accumulation of p21 and an increase in senescence-associated β-galactosidase activity. In contrast, inhibition of REV3 expression in p53-deficient cells results in growth inhibition and a G(2)/M arrest. A small fraction of the p53-deficient cancer cells can overcome the G(2)/M arrest, which results in mitotic slippage and aneuploidy. Our findings reveal that REV3 depletion per se suppresses growth of cancer cell lines from different origin, whereas control cell lines and a mesothelial primary culture were less affected. Thus, our findings indicate that depletion of REV3 not only can amend cisplatin-based cancer therapy but also can be applied for susceptible cancers as a potential monotherapy.
Keywords
Ataxia Telangiectasia Mutated Proteins, Blotting, Western, Cell Aging, Cell Cycle Checkpoints, Cell Cycle Proteins/metabolism, Cell Division, Cell Line, Tumor, Cell Proliferation, DNA Breaks, Double-Stranded, DNA Damage, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, DNA-Directed DNA Polymerase/genetics, DNA-Directed DNA Polymerase/metabolism, G2 Phase, Genomic Instability, HCT116 Cells, HEK293 Cells, Histones/metabolism, Humans, Intracellular Signaling Peptides and Proteins/metabolism, Microscopy, Fluorescence, Neoplasms/genetics, Neoplasms/metabolism, Protein-Serine-Threonine Kinases/metabolism, RNA Interference, Tumor Suppressor Protein p53/metabolism, Tumor Suppressor Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
03/08/2014 14:39
Last modification date
08/05/2019 21:48
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