Effect of conditioned media, nutritive elements, and mitotic synchronization on the accuracy of the cytogenetic analysis in acute nonlymphocytic leukemia patients presenting with inv(16)/t(16;16) or t(15;17).

Details

Serval ID
serval:BIB_8C881F4EAE0F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effect of conditioned media, nutritive elements, and mitotic synchronization on the accuracy of the cytogenetic analysis in acute nonlymphocytic leukemia patients presenting with inv(16)/t(16;16) or t(15;17).
Journal
Cancer Genetics and Cytogenetics
Author(s)
Castagné C., Mühlematter D., van Melle G., Gachoud V., Jotterand Bellomo M.
ISSN
0165-4608
Publication state
Published
Issued date
1997
Volume
94
Number
2
Pages
106-112
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
To improve the yield of the cytogenetic analysis in patients with acute nonlymphocytic leukemia (ANLL), six culture conditions for bone marrow or peripheral blood cells were tested in parallel. Two conditioned media (CM), phytohemagglutinin leukocyte PHA-LCM and 5637 CM, nutritive elements (NE), and methotrexate (MTX) cell synchronization were investigated in 14 patients presenting with either inv(16)/ t(16;16) (group 1, n = 9 patients) or t(15;17) (group 2, n = 5). The criteria used to identify the most favorable culture conditions were the mitotic index (MI), the morphological index (MorI), and the percentage of abnormal metaphases. In the presence of PHA-LCM and 5637 CM, the MI were significantly increased in group 2, whereas in the MTX conditions, MI remained very low in both groups. The values of the MorI did not reveal any significant changes in chromosome resolution between the conditions in either group. The addition of NE did not have a positive effect in quantity or quality of metaphases. Because of the variability of the response of leukemic cells to different stimulations in vitro, several culture conditions in parallel are required to ensure a satisfactory yield of the chromosome analysis in ANLL.
Keywords
Bone Marrow, Chromosome Aberrations, Chromosome Disorders, Chromosomes, Human, Pair 15, Chromosomes, Human, Pair 16, Chromosomes, Human, Pair 17, Culture Media, Cytogenetics, Humans, Inversion, Chromosome, Leukemia, Myeloid, Acute, Mitosis, Translocation, Genetic, Tumor Cells, Cultured
Pubmed
Web of science
Create date
22/05/2009 10:16
Last modification date
20/08/2019 15:50
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