Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Details
Serval ID
serval:BIB_8C31DA27523F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Journal
PLoS biology
ISSN
1545-7885 (Electronic)
ISSN-L
1544-9173
Publication state
Published
Issued date
12/2024
Peer-reviewed
Oui
Volume
22
Number
12
Pages
e3002963
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
Starvation, which is associated with inactivation of the growth-promoting TOR complex 1 (TORC1), is a strong environmental signal for cell differentiation. In the fission yeast Schizosaccharomyces pombe, nitrogen starvation has distinct physiological consequences depending on the presence of mating partners. In their absence, cells enter quiescence, and TORC1 inactivation prolongs their life. In presence of compatible mates, TORC1 inactivation is essential for sexual differentiation. Gametes engage in paracrine pheromone signaling, grow towards each other, fuse to form the diploid zygote, and form resistant, haploid spore progenies. To understand the signaling changes in the proteome and phospho-proteome during sexual reproduction, we developed cell synchronization strategies and present (phospho-)proteomic data sets that dissect pheromone from starvation signals over the sexual differentiation and cell-cell fusion processes. Unexpectedly, these data sets reveal phosphorylation of ribosomal protein S6 during sexual development, which we establish requires TORC1 activity. We demonstrate that TORC1 is re-activated by pheromone signaling, in a manner that does not require autophagy. Mutants with low TORC1 re-activation exhibit compromised mating and poorly viable spores. Thus, while inactivated to initiate the mating process, TORC1 is reactivated by pheromone signaling in starved cells to support sexual reproduction.
Keywords
Schizosaccharomyces/metabolism, Schizosaccharomyces/genetics, Schizosaccharomyces/physiology, Pheromones/metabolism, Signal Transduction, Proteomics/methods, Schizosaccharomyces pombe Proteins/metabolism, Schizosaccharomyces pombe Proteins/genetics, Mechanistic Target of Rapamycin Complex 1/metabolism, Phosphorylation, Reproduction/physiology, Proteome/metabolism, Phosphoproteins/metabolism, Gene Expression Regulation, Fungal, Spores, Fungal/metabolism, Spores, Fungal/physiology
Pubmed
Web of science
Open Access
Yes
Create date
08/01/2025 15:19
Last modification date
28/01/2025 7:06