Improved polymerase chain reaction detection of clonal T-cell lymphoid neoplasms.

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_8BD1DAF967B5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Improved polymerase chain reaction detection of clonal T-cell lymphoid neoplasms.
Journal
Diagnostic molecular pathology
Author(s)
Benhattar J., Delacretaz F., Martin P., Chaubert P., Costa J.
ISSN
1052-9551 (Print)
ISSN-L
1052-9551
Publication state
Published
Issued date
06/1995
Peer-reviewed
Oui
Volume
4
Number
2
Pages
108-112
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
We have developed and tested a rapid and sensitive method of detecting expansion of T-cell clones using the polymerase chain reaction (PCR) and a single set of consensus primers for the V and J regions to amplify rearranged T-cell receptor-gamma (TCR-gamma) genes. Monoclonality was continued in all of the 18 cases of T-cell neoplasms tested, but not in reactive lymphadenopathy, non-Hodgkin's B lymphomas, and Hodgkin's disease. PCR analysis, using the primer sequence outlined in this study, had an overall specificity of 100% when compared with Southern blot analysis. No false-negative results were observed, certainly owing to the choice of consensus primers and to the control of PCR reactions on agarose gels before testing for clonality by separation of PCR products on polyacrylamide gels. This method for the detection of T-cell monoclonality can be especially useful in cases that are diagnostically problematic with standard histological and immunological analysis and in cases where the material available is limited.
Keywords
Base Sequence, Blotting, Southern, Clone Cells/pathology, Humans, Immunohistochemistry, Lymphoma, T-Cell/diagnosis, Lymphoma, T-Cell/genetics, Lymphoma, T-Cell/pathology, Molecular Sequence Data, Polymerase Chain Reaction/methods, Receptors, Antigen, T-Cell, gamma-delta/analysis, Sensitivity and Specificity
Pubmed
Web of science
Create date
29/01/2008 19:35
Last modification date
01/08/2020 6:19
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