Sex chromosome and sex locus characterization in goldfish, Carassius auratus (Linnaeus, 1758)

Details

Serval ID
serval:BIB_8A2D03FDA328
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sex chromosome and sex locus characterization in goldfish, Carassius auratus (Linnaeus, 1758)
Journal
BMC Genomics
Author(s)
Wen Ming, Feron Romain, Pan Qiaowei, Guguin Justine, Jouanno Elodie, Herpin Amaury, Klopp Christophe, Cabau Cedric, Zahm Margot, Parrinello Hugues, Journot Laurent, Burgess Shawn M., Omori Yoshihiro, Postlethwait John H., Schartl Manfred, Guiguen Yann
ISSN
1471-2164 (Electronic)
ISSN-L
1471-2164
Publication state
Published
Issued date
12/2020
Peer-reviewed
Oui
Volume
21
Number
1
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Goldfish is an important model for various areas of research, including neural development and behavior and a species of significant importance in aquaculture, especially as an ornamental species. It has a male heterogametic (XX/XY) sex determination system that relies on both genetic and environmental factors, with high temperatures being able to produce female-to-male sex reversal. Little, however, is currently known on the molecular basis of genetic sex determination in this important cyprinid model. Here we used sequencing approaches to better characterize sex determination and sex-chromosomes in an experimental strain of goldfish.
Our results confirmed that sex determination in goldfish is a mix of environmental and genetic factors and that its sex determination system is male heterogametic (XX/XY). Using reduced representation (RAD-seq) and whole genome (pool-seq) approaches, we characterized sex-linked polymorphisms and developed male specific genetic markers. These male specific markers were used to distinguish sex-reversed XX neomales from XY males and to demonstrate that XX female-to-male sex reversal could even occur at a relatively low rearing temperature (18 °C), for which sex reversal has been previously shown to be close to zero. We also characterized a relatively large non-recombining region (~ 11.7 Mb) on goldfish linkage group 22 (LG22) that contained a high-density of male-biased genetic polymorphisms. This large LG22 region harbors 373 genes, including a single candidate as a potential master sex gene, i.e., the anti-Mullerian hormone gene (amh). However, no sex-linked polymorphisms were detected in the coding DNA sequence of the goldfish amh gene.
These results show that our goldfish strain has a relatively large sex locus on LG22, which is likely the Y chromosome of this experimental population. The presence of a few XX males even at low temperature also suggests that other environmental factors in addition to temperature could trigger female-to-male sex reversal. Finally, we also developed sex-linked genetic markers, which will be important tools for future research on sex determination in our experimental goldfish population. However, additional work would be needed to explore whether this sex locus is conserved in other populations of goldfish.
Keywords
Biotechnology, Genetics
Pubmed
Open Access
Yes
Create date
13/08/2020 15:14
Last modification date
29/08/2020 6:20
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