Aqueous dynamic and histological findings after deep sclerectomy with collagen implant in an animal model.

Details

Serval ID
serval:BIB_8A0275F234E8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Aqueous dynamic and histological findings after deep sclerectomy with collagen implant in an animal model.
Journal
British Journal of Ophthalmology
Author(s)
Delarive T., Rossier A., Rossier S., Ravinet E., Shaarawy T., Mermoud A.
ISSN
0007-1161
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
87
Number
11
Pages
1340-1344
Language
english
Notes
Publication types: Journal Article
Abstract
AIM: The use of an animal model to study the aqueous dynamic and the histological findings after deep sclerectomy with (DSCI) and without collagen implant. METHODS: Deep sclerectomy was performed on rabbits' eyes. Eyes were randomly assigned to receive collagen implants. Measurements of intraocular pressure (IOP) and aqueous outflow facility using the constant pressure method through cannulation of the anterior chamber were performed. The system was filled with BSS and cationised ferritin. Histological assessment of the operative site was performed. Sections were stained with haematoxylin and eosin and with Prussian blue. Aqueous drainage vessels were identified by the reaction between ferritin and Prussian blue. All eyes were coded so that the investigator was blind to the type of surgery until the evaluation was completed. RESULTS: A significant decrease in IOP (p<0.05) was observed during the first 6 weeks after DSCI (mean IOP was 13.07 (2.95) mm Hg preoperatively and 9.08 (2.25) mm Hg at 6 weeks); DS without collagen implant revealed a significant decrease in IOP at weeks 4 and 8 after surgery (mean IOP 12.57 (3.52) mm Hg preoperatively, 9.45 (3.38) mm Hg at 4 weeks, and 9.22 (3.39) mm Hg at 8 weeks). Outflow facility was significantly increased throughout the 9 months of follow up in both DSCI and DS groups (p<0.05). The preoperative outflow facility (OF) was 0.15 (0.02) micro l/min/mm Hg. At 9 months, OF was 0.52 (0.28) microl/min/mm Hg and 0.46 (0.07) micro l/min/mm Hg for DSCI and DS respectively. Light microscopy studies showed the appearance of new aqueous drainage vessels in the sclera adjacent to the dissection site in DSCI and DS and the apparition of spindle cells lining the collagen implant in DSCI after 2 months. CONCLUSION: A significant IOP decrease was observed during the first weeks after DSCI and DS. DS with or without collagen implant provided a significant increase in outflow facility throughout the 9 months of follow up. This might be partly explained by new drainage vessels in the sclera surrounding the operated site. Microscopic studies revealed the appearance of spindle cells lining the collagen implant in DSCI after 2 months.
Keywords
Animals, Aqueous Humor, Collagen, Female, Glaucoma, Glaucoma Drainage Implants, Intraocular Pressure, Models, Animal, Rabbits, Sclerostomy
Pubmed
Web of science
Open Access
Yes
Create date
28/02/2008 14:01
Last modification date
20/08/2019 15:48
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