Altered control of glutamate dehydrogenases in ornithine utilization mutants of Pseudomonas aeruginosa.
Details
Serval ID
serval:BIB_89172B5E9F98
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Altered control of glutamate dehydrogenases in ornithine utilization mutants of Pseudomonas aeruginosa.
Journal
Archives of Microbiology
ISSN
0302-8933 (Print)
ISSN-L
0302-8933
Publication state
Published
Issued date
1985
Volume
141
Number
2
Pages
170-176
Language
english
Abstract
Two classes of ornithine-nonutilizing (oru) mutants of Pseudomonas aeruginosa PAO were investigated. Strains carrying the oru-310 mutation were entirely unable to grow on L-ornithine as the only carbon and nitrogen source and were affected in the assimilation of a variety of nitrogen sources (e.g., amino acids, nitrate). The oru-310 mutation caused changes in the regulation of the catabolic NAD-dependent glutamate dehydrogenase; this enzyme was no longer inducible by glutamate but instead could be induced by ammonia. The oru-310 locus was cotransducible with car-9 and tolA in the 10 min region of the chromosome. An oru-314 mutant was severely handicapped in ornithine medium but could grow when a good carbon source was added; the mutant also showed pleiotropic growth effects related to nitrogen metabolism. The oru-314 mutation affected the regulation of the anabolic NADP-dependent glutamate dehydrogenase, which was no longer repressed by glutamate but showed normal derepression in the presence of ammonia. The oru-314 locus was mapped by transduction near met-9011 at 55 min. Both oru mutants could grow on L-glutamate, L-proline, or L-ornithine amended with 2-oxoglutarate, albeit slowly. We speculate that insufficient 2-oxoglutarate concentrations might account, at least in part, for the Oru- phenotype of the mutants.
Keywords
Chromosome Mapping, Glutamate Dehydrogenase/genetics, Glutamates/metabolism, Glutamic Acid, Mutation, Ornithine/metabolism, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics
Pubmed
Web of science
Create date
25/01/2008 17:01
Last modification date
20/08/2019 14:48