Induction of apoptosis by p75 neurotrophin receptor in human neuroblastoma cells

Details

Serval ID
serval:BIB_890A028F6B89
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Induction of apoptosis by p75 neurotrophin receptor in human neuroblastoma cells
Journal
Oncogene
Author(s)
Bunone  G., Mariotti  A., Compagni  A., Morandi  E., Della Valle  G.
ISSN
0950-9232 (Print)
Publication state
Published
Issued date
03/1997
Volume
14
Number
12
Pages
1463-70
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 27
Abstract
The low-affinity nerve growth factor receptor p75NTR belongs to a membrane receptor superfamily whose members, in certain cell types, are able to transduce an apoptotic signal. To investigate the effect of p75NTR expression in neuroblastoma cells, we transfected the p75NTR cDNA into SK-N-BE cells, a neuroblastoma cell line that lacks expression of both p75NTR and TrkA. Cell clones expressing elevated levels of p75NTR showed a high degree of cell death by apoptosis, even in serum-supplemented medium. Moreover, the level of apoptosis correlated directly with the expression level of the receptor, indicating that p75NTR could activate the cell death program by itself. Clones expressing p75NTR showed a dramatic increase of cell death when switched into serum-free medium; these cultures rapidly extinguished. This apoptotic effect was greatly inhibited by NGF treatment. Our results support the hypothesis that p75NTR, when it is not bound by NGF, may play a role in neuronal selection during embryonic development and suggest that neuroblastomas may arise from immature neuroblasts that escape programmed cell death. Therefore, the loss of p75NTR expression in developing neural crest cells might be a primary event in the genesis of neuroblastoma.
Keywords
*Apoptosis Cell Survival/drug effects Gene Expression Humans Nerve Growth Factors/pharmacology Neuroblastoma Neurons/*cytology RNA, Messenger/genetics Receptor, Nerve Growth Factor Receptors, Nerve Growth Factor/*physiology Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 8:34
Last modification date
20/08/2019 14:48
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