Alterations of Subchondral Bone Progenitor Cells in Human Knee and Hip Osteoarthritis Lead to a Bone Sclerosis Phenotype.

Details

Ressource 1Download: 29415458_BIB_88D37611046A.pdf (18703.22 [Ko])
State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_88D37611046A
Type
Article: article from journal or magazin.
Publication sub-type
Minutes: analyse of a published work.
Collection
Publications
Institution
Title
Alterations of Subchondral Bone Progenitor Cells in Human Knee and Hip Osteoarthritis Lead to a Bone Sclerosis Phenotype.
Journal
International journal of molecular sciences
Author(s)
Bianco D., Todorov A., Čengić T., Pagenstert G., Schären S., Netzer C., Hügle T., Geurts J.
ISSN
1422-0067 (Electronic)
ISSN-L
1422-0067
Publication state
Published
Issued date
06/02/2018
Peer-reviewed
Oui
Volume
19
Number
2
Pages
475
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Subchondral bone tissue plays a key role in the initiation and progression of human and experimental osteoarthritis and has received considerable interest as a treatment target. Elevated bone turnover and remodeling leads to subchondral bone sclerosis that is characterized by an increase in bone material that is less mineralized. The aim of this study was to investigate whether perturbations in subchondral bone-resident progenitor cells might play a role in aberrant bone formation in osteoarthritis. Colony formation assays indicated similar clonogenicity of progenitor cells from non-sclerotic and sclerotic subchondral trabecular bone tissues of osteoarthritic knee and hip joints compared with controls from iliac crest bone. However, the osteogenic potential at the clonal level was approximately two-fold higher in osteoarthritis than controls. An osteogenic differentiation assay indicated an efficient induction of alkaline phosphatase activity but blunted in vitro matrix mineralization irrespective of the presence of sclerosis. Micro-computed tomography and histology demonstrated the formation of de novo calcified tissues by osteoblast-like cells in an ectopic implantation model. The expression of bone sialoprotein, a marker for osteoblast maturation and mineralization, was significantly less in sclerotic progenitor cells. Perturbation of resident progenitor cell function is associated with subchondral bone sclerosis and may be a treatment target for osteoarthritis.
Keywords
clonogenicity, computed tomography, ectopic bone formation, osteoarthritis, osteogenic differentiation, osteoprogenitors, subchondral bone
Pubmed
Web of science
Open Access
Yes
Create date
23/02/2018 13:14
Last modification date
28/07/2020 6:21
Usage data