Hepatic ischemia-reperfusion increases circulating bone marrow-derived progenitor cells and tumor growth in a mouse model of colorectal liver metastases.

Details

Serval ID
serval:BIB_881D6E14E53D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Hepatic ischemia-reperfusion increases circulating bone marrow-derived progenitor cells and tumor growth in a mouse model of colorectal liver metastases.
Journal
Journal of Surgical Research
Author(s)
Lim C., Broqueres-You D., Brouland J.P., Merkulova-Rainon T., Faussat A.M., Hilal R., Rouquie D., Eveno C., Audollent R., Levy B.I., Pocard M.
ISSN
1095-8673 (Electronic)
ISSN-L
0022-4804
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
184
Number
2
Pages
888-897
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
BACKGROUND: Hepatic pedicle clamping is often required to reduce blood loss and transfusion during liver resection. However, the question remains whether use of hepatic pedicle clamping promotes tumor growth. Endothelial progenitor cells (EPCs) are mobilized from bone marrow in response to tissue ischemia, which allows neovascularization of ischemic tissue. It has been suggested that EPCs are involved in tumor progression. We hypothesized that hepatic ischemia reperfusion (I/R)-induced mobilization of EPCs could enhance growth of microscopic tumor, therefore promoting liver metastasis in a mouse model of colorectal cancer.
MATERIALS AND METHODS: We used mouse models of hepatic I/R and hind limb ischemia. For comparison, we studied mice that underwent limb ischemia as positive controls of EPC mobilization. At day 0, we divided 40 mice into four groups: hepatic I/R, hind limb ischemia, combined hepatic I/R and hind limb ischemia, and control (sham midline incision laparotomy). At day 2, we induced liver metastasis in all mice by injecting CT-26 cells into the spleen. Time-dependent circulating EPCs were determined by flow cytometry. We evaluated liver metastasis and microvascular density on day 21.
RESULTS: The number of circulating progenitor cells increased rapidly in the ischemic groups compared with the control group. Hepatic I/R significantly increased tumor outgrowth compared with the control group. Increased tumor growth was associated with enhanced CD31-positive microvascular density in liver tissue.
CONCLUSIONS: Hepatic I/R leads to mobilization of bone marrow-derived EPCs and enhanced intra-hepatic angiogenesis, which is associated with increased tumor burden in an animal model of colorectal liver metastasis.
Keywords
Animals, Bone Marrow Cells/pathology, Cell Count, Cell Line, Tumor, Cell Proliferation, Chemokine CXCL12/blood, Colorectal Neoplasms/pathology, Disease Models, Animal, Disease Progression, Female, Hematopoietic Stem Cells/pathology, Liver/blood supply, Liver Neoplasms/pathology, Liver Neoplasms/secondary, Mice, Mice, Inbred BALB C, Neoplasm Metastasis/physiopathology, Neovascularization, Pathologic/physiopathology, Reperfusion Injury/physiopathology
Pubmed
Web of science
Create date
13/10/2015 10:17
Last modification date
20/08/2019 14:47
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