Improved neoangiogenesis in transmyocardial laser revascularization combined with angiogenic adjunct in a pig model.

Details

Serval ID
serval:BIB_876015BD432B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Improved neoangiogenesis in transmyocardial laser revascularization combined with angiogenic adjunct in a pig model.
Journal
Clinical Science
Author(s)
Mueller X.M., Tevaearai H.T., Genton C.Y., Chaubert P., von Segesser L.K.
ISSN
0143-5221
Publication state
Published
Issued date
12/2000
Peer-reviewed
Oui
Volume
99
Number
6
Pages
535-540
Language
english
Abstract
Recent research has revealed neoangiogenesis as a basic phenomenon in transmyocardial laser revascularization (TMLR). Theoretically, myocardial neoangiogenesis could be further enhanced by the addition of angiogenic growth factors. Triads of TMLR channels were created in the lateral wall of the left ventricles of 12 pigs (mean body weight 73+/-5 kg), using a holmium:yttrium-aluminium garnet (YAG) laser. The animals were allocated randomly either to receive an injection of 100 microg of a bovine bone-derived growth factor mixture within the triads (n=6), or to a control group (n=6). Animals were killed 1 month later. Capillary and arteriolar densities were determined by computed morphometric analysis of histological sections of the triads. The capillary density of myocardial areas within the triads was significantly greater in the group receiving the bovine bone-derived growth factor mixture than in the control group (14.3+/-3.5/mm(2) and 5.7+/-1.4/mm(2) respectively; P<0.001). The difference was also significant when considering arteriolar density (0.7+/-0.4/mm(2) and 0.2+/-0.1/mm(2) respectively; P<0.001). For comparison, capillary and arteriolar densities of the TMLR channel scars were 48.7+/-9.7 and 1.9+/-0. 5/mm(2) respectively in the angiogenic group, and 46.3+/-13.7 and 2. 3+/-1.3/mm(2) respectively in the control group (no significant differences). These results demonstrate that the addition of angiogenic factors to TMLR stimulates neoangiogenesis significantly in the areas adjacent to the channels, but not within the channel scars. The latter are themselves strongly vascularized. Hence this combined approach, potentiating the effect of TMLR by establishing vascular connections between the neovessels of the channel scars, has the potential for improved clinical outcome.
Keywords
Animals, Growth Substances/pharmacology, Growth Substances/therapeutic use, Laser Therapy/methods, Myocardial Revascularization/methods, Myocardium/ultrastructure, Neovascularization, Physiologic/drug effects, Swine
Pubmed
Web of science
Create date
14/02/2008 15:16
Last modification date
20/08/2019 15:46
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