cDNA and protein characterization of human MAGE-10
Details
Serval ID
serval:BIB_8757850ADF29
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
cDNA and protein characterization of human MAGE-10
Journal
International Journal of Cancer
ISSN
0020-7136 (Print)
Publication state
Published
Issued date
09/1999
Volume
82
Number
6
Pages
901-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 9
Research Support, Non-U.S. Gov't --- Old month value: Sep 9
Abstract
MAGE genes are frequently expressed in several types of human malignancy and code for antigens recognized by cytotoxic T lymphocytes. We have previously described a monoclonal antibody (MAb), named 6C1, that recognizes the MAGE-1 protein and cross-reacts with a 72-kDa protein present in lysates of melanoma cells such as MZ2-MEL. To identify this protein, we have screened an expression library prepared from MZ2-MEL cells. Several clones that encoded a protein recognized by antibody 6C1 contained a sequence identical to that of MAGE-10, another member of the MAGE-A gene family. Full-length MAGE-10 cDNA clones, obtained after screening additional cDNA melanoma libraries, were found to be approximately 2.5 kb in length. In vitro translation and transient transfection experiments indicated that MAGE-10 codes for a protein of approximately 72 kDa. This product was recognized by MAb 6C1 as well as by a polyclonal serum raised against a MAGE-10 peptide, thus demonstrating its identity with MAGE-10. Analysis of MAGE-10 mRNA by RT-PCR confirmed its presence in testis and placenta but not in other normal tissues. Expression of MAGE-10 in melanoma tumors was found to parallel that of MAGE-1. Western blot analysis with the polyclonal anti-MAGE-10 antibody showed the presence of MAGE-10 in lysates of purified trophoblast cells. Immuno-cytochemistry of cultured melanoma cells indicated that MAGE-10 is a nuclear protein.
Keywords
Antibodies, Monoclonal
Antigens, Neoplasm/*genetics
Blotting, Northern
Cloning, Molecular
DNA, Complementary
Female
Gene Library
Humans
Introns
Male
Melanoma/*genetics
Neoplasm Proteins/chemistry/*genetics/metabolism
Organ Specificity
Polymerase Chain Reaction
Protein Biosynthesis
Recombinant Proteins/biosynthesis/chemistry/metabolism
Transcription, Genetic
Transfection
Pubmed
Web of science
Create date
28/01/2008 12:13
Last modification date
20/08/2019 15:46