Identification of the gonad-specific anion transporter SLCO6A1 as a cancer/testis (CT) antigen expressed in human lung cancer.

Details

Serval ID
serval:BIB_872E597D0B15
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of the gonad-specific anion transporter SLCO6A1 as a cancer/testis (CT) antigen expressed in human lung cancer.
Journal
Cancer Immunity
Author(s)
Lee S.Y., Williamson B., Caballero O.L., Chen Y.T., Scanlan M.J., Ritter G., Jongeneel C.V., Simpson A.J., Old L.J.
ISSN
1424-9634[electronic], 1424-9634[linking]
Publication state
Published
Issued date
2004
Volume
4
Pages
13
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Serological analysis of recombinant cDNA expression libraries (SEREX) has led to the identification of many of the antigens recognized by the immune system of cancer patients, which are collectively referred to as the cancer immunome. We used SEREX to screen a testicular cDNA expression library with sera obtained from non-small cell lung cancer patients and isolated cDNA clones for 82 antigens. These included a total of 31 antigens previously identified by SEREX, and 51 that did not match entries in the Cancer Immunome Database and were considered newly identified antigens. Overall, the antigens comprised 62 known proteins and 20 uncharacterized gene products. Six antigens (NY-TLU-6, -37, -39, -57, -70, -75) were identified as putative cell surface proteins that are potential targets for monoclonal antibody-based immunotherapy. Of these, the gonad-specific anion transport protein SLCO6A1 (NY-TLU-57) was shown to be tissue-restricted. RT-PCR showed it to be expressed strongly only in normal testis, and weakly in spleen, brain, fetal brain, and placenta. In addition, NY-TLU-57 mRNA was found in lung tumor samples (5/10) and lung cancer cell lines (6/11), as well as bladder (5/12) and esophageal (5/12) tumor samples. These data suggest that SLCO6A1 is a putative cancer/testis (CT) cell surface antigen of potential utility as a target for antibody-based therapy for a variety of tumor types. The analysis also permits us to estimate the eventual size of the SEREX-defined cancer immunome at around 4000 genes. This emphasizes the importance of continued SEREX screening to define the cancer immunome.
Keywords
Anion Transport Proteins/biosynthesis, Anion Transport Proteins/genetics, Antigens, Neoplasm/biosynthesis, Antigens, Neoplasm/genetics, Carcinoma, Non-Small-Cell Lung/blood, Carcinoma, Non-Small-Cell Lung/genetics, DNA, Neoplasm/blood, DNA, Neoplasm/genetics, Gene Library, Humans, Lung Neoplasms/blood, Lung Neoplasms/genetics, Male, RNA, Messenger/biosynthesis, RNA, Messenger/genetics, Testis/immunology
Pubmed
Create date
24/01/2008 15:40
Last modification date
20/08/2019 14:46
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