A novel protein kinase that controls carbon catabolite repression in bacteria

Details

Serval ID
serval:BIB_871360201FFA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A novel protein kinase that controls carbon catabolite repression in bacteria
Journal
Molecular Microbiology
Author(s)
Reizer  J., Hoischen  C., Titgemeyer  F., Rivolta  C., Rabus  R., Stulke  J., Karamata  D., Saier, M. H., Jr. , Hillen  W.
ISSN
0950-382X (Print)
Publication state
Published
Issued date
03/1998
Peer-reviewed
Oui
Volume
27
Number
6
Pages
1157-69
Notes
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Abstract
HPr(Ser) kinase is the sensor in a multicomponent phosphorelay system that controls catabolite repression, sugar transport and carbon metabolism in gram-positive bacteria. Unlike most other protein kinases, it recognizes the tertiary structure in its target protein, HPr, a phosphocarrier protein of the bacterial phosphotransferase system and a transcriptional cofactor controlling the phenomenon of catabolite repression. We have identified the gene (ptsK) encoding this serine/threonine protein kinase and characterized the purified protein product. Orthologues of PtsK have been identified only in bacteria. These proteins constitute a novel family unrelated to other previously characterized protein phosphorylating enzymes. The Bacillus subtilis kinase is shown to be allosterically activated by metabolites such as fructose 1,6-bisphosphate and inhibited by inorganic phosphate. In contrast to wild-type B. subtilis, the ptsK mutant is insensitive to transcriptional regulation by catabolite repression. The reported results advance our understanding of phosphorylation-dependent carbon control mechanisms in Gram-positive bacteria.
Keywords
Adenosine Triphosphate/pharmacology Allosteric Regulation/physiology Amino Acid Sequence Bacillus subtilis/*enzymology/metabolism Bacterial Proteins/chemistry Enzyme Activation/physiology Enzyme Inhibitors Escherichia coli/genetics Fructosediphosphates/pharmacology Genome, Bacterial Molecular Sequence Data Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism Phosphorylation Protein Kinase Inhibitors Protein Kinases/*chemistry *Protein-Serine-Threonine Kinases Recombinant Proteins/chemistry Sequence Alignment Sequence Analysis
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:14
Last modification date
20/08/2019 14:46
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