A novel protein kinase that controls carbon catabolite repression in bacteria
Details
Serval ID
serval:BIB_871360201FFA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A novel protein kinase that controls carbon catabolite repression in bacteria
Journal
Molecular Microbiology
ISSN
0950-382X (Print)
Publication state
Published
Issued date
03/1998
Peer-reviewed
Oui
Volume
27
Number
6
Pages
1157-69
Notes
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Abstract
HPr(Ser) kinase is the sensor in a multicomponent phosphorelay system that controls catabolite repression, sugar transport and carbon metabolism in gram-positive bacteria. Unlike most other protein kinases, it recognizes the tertiary structure in its target protein, HPr, a phosphocarrier protein of the bacterial phosphotransferase system and a transcriptional cofactor controlling the phenomenon of catabolite repression. We have identified the gene (ptsK) encoding this serine/threonine protein kinase and characterized the purified protein product. Orthologues of PtsK have been identified only in bacteria. These proteins constitute a novel family unrelated to other previously characterized protein phosphorylating enzymes. The Bacillus subtilis kinase is shown to be allosterically activated by metabolites such as fructose 1,6-bisphosphate and inhibited by inorganic phosphate. In contrast to wild-type B. subtilis, the ptsK mutant is insensitive to transcriptional regulation by catabolite repression. The reported results advance our understanding of phosphorylation-dependent carbon control mechanisms in Gram-positive bacteria.
Keywords
Adenosine Triphosphate/pharmacology Allosteric Regulation/physiology Amino Acid Sequence Bacillus subtilis/*enzymology/metabolism Bacterial Proteins/chemistry Enzyme Activation/physiology Enzyme Inhibitors Escherichia coli/genetics Fructosediphosphates/pharmacology Genome, Bacterial Molecular Sequence Data Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism Phosphorylation Protein Kinase Inhibitors Protein Kinases/*chemistry *Protein-Serine-Threonine Kinases Recombinant Proteins/chemistry Sequence Alignment Sequence Analysis
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:14
Last modification date
20/08/2019 15:46