Automated electron tomography of the septal pore cap in Rhizoctonia solani.

Details

Serval ID
serval:BIB_870DEDE24597
Type
Article: article from journal or magazin.
Collection
Publications
Title
Automated electron tomography of the septal pore cap in Rhizoctonia solani.
Journal
Journal of Structural Biology
Author(s)
Müller W.H., Koster A.J., Humbel B.M., Ziese U., Verkleij A.J., van Aelst A.C., van der Krift T.P., Montijn R.C., Boekhout T.
ISSN
1047-8477 (Print)
ISSN-L
1047-8477
Publication state
Published
Issued date
2000
Volume
131
Number
1
Pages
10-18
Language
english
Abstract
Dolipore septa and septal pore caps (SPCs) in filamentous basidiomycetes may play an important role in maintaining the integrity of hyphal cells. We have investigated the ultrastructure of the dolipore septum and the SPC in Rhizoctonia solani hyphal cells after high-pressure freezing, freeze substitution, and Spurr embedding. We visualized the SPC with associated cell ultrastructures in three dimensions by automated electron tomography of thick-sectioned cells, followed by 3D tomographic reconstructions. Using these methods we were able to document the passage of mitochondria through the SPC, small tubular membranous structures at the entrance of the septal pore channel, filamentous structures connecting the inner side of the SPC with pore-plugging material, thin filaments anchoring the pore-plugging material with the plasma membrane, small vesicles attached to the plugging material, and tubular endoplasmic reticulum continuous with the base of the SPC. We hypothesize that the SPC, the filamentous structures, the plugging material, and the endoplasmic reticulum act in a coordinated fashion to maintain cellular integrity, intercellular communication, and the transport of solutes and cell organelles in the filamentous fungus R. solani.
Keywords
Automation, Cell Membrane/metabolism, Cell Membrane/ultrastructure, Cryoelectron Microscopy, Electrons, Endoplasmic Reticulum/metabolism, Endoplasmic Reticulum/ultrastructure, Freezing, Image Processing, Computer-Assisted, Intercellular Junctions/metabolism, Intercellular Junctions/ultrastructure, Intracellular Membranes/metabolism, Intracellular Membranes/ultrastructure, Microscopy, Electron, Microscopy, Electron, Scanning, Mitochondria/metabolism, Mitochondria/ultrastructure, Rhizoctonia/cytology, Rhizoctonia/ultrastructure, Tomography
Pubmed
Web of science
Create date
18/10/2012 14:09
Last modification date
20/08/2019 14:46
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