Characterization of an antibody panel for immunohistochemical analysis of canine muscle cells.

Details

Serval ID
serval:BIB_870A002DFE58
Type
Article: article from journal or magazin.
Collection
Publications
Title
Characterization of an antibody panel for immunohistochemical analysis of canine muscle cells.
Journal
Veterinary Immunology and Immunopathology
Author(s)
Gofflot S., Kischel P., Thielen C., Radermacher V., Boniver J., de Leval L.
ISSN
0165-2427[print], 0165-2427[linking]
Publication state
Published
Issued date
2008
Volume
125
Number
3-4
Pages
225-233
Language
english
Abstract
Immunohistochemistry is an indispensable tool in the assessment and characterization of lineage-specific differentiation of grafted cells in cell-based-therapy. This strategy is under investigation for the treatment of many muscle disorders and different animals such as dogs are used as models to study the tissue regeneration. The aim of the present study was to characterize an antibody panel for the analysis of canine muscle cells, useful in routinely processed formalin-fixed paraffin-embedded tissues. Overall, 12 antibodies (8 mouse monoclonal and 4 goat polyclonal), validated for use on human tissues tested for cross-reactivity on canine smooth muscle (bladder, intestine, and uterus), skeletal muscle and heart. Specific staining was achieved with eight antibodies, of which six were cytoplasmic markers (desmin, HDAC8, MHC, SMA, Troponin I and Troponin T) and two were cardiac nuclear markers (GATA-4 and Nkx-2.5). This antibody panel may be useful not only for the evaluation of cell-based therapies in muscle disorders, but also for the evaluation of canine soft tissue neoplasms in veterinary pathology.
Keywords
Animals, Antibodies/chemistry, Antibodies/immunology, Blotting, Western/veterinary, Cell Differentiation/physiology, Dogs/physiology, Female, Immunohistochemistry/methods, Immunohistochemistry/veterinary, Male, Muscle, Smooth/chemistry, Muscle, Smooth/cytology, Myocytes, Smooth Muscle/chemistry
Pubmed
Create date
27/10/2010 8:43
Last modification date
20/08/2019 14:46
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