Interleukin 4 down-regulates expression of c-kit and autocrine stem cell factor in human colorectal carcinoma cells

Details

Serval ID
serval:BIB_868DCE12B8BA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Interleukin 4 down-regulates expression of c-kit and autocrine stem cell factor in human colorectal carcinoma cells
Journal
Cell Growth and Differentiation
Author(s)
Lahm  H., Amstad  P., Yilmaz  A., Borbenyi  Z., Wyniger  J., Fischer  J. R., Suardet  L., Givel  J. C., Odartchenko  N.
ISSN
1044-9523 (Print)
Publication state
Published
Issued date
09/1995
Volume
6
Number
9
Pages
1111-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Abstract
Stem cell factor (SCF) is a cytokine which plays an important role in the development of precursor cells. We have investigated the expression of SCF and its receptor, the c-kit proto-oncogene, in human colorectal carcinoma cell lines. Using reverse transcription-PCR, we confirmed the expression of c-kit in two lines (LS174T and LS1034) and of SCF in 9 of 11 cell lines tested. In a Northern blot, a single transcript of 6.6 kb was detected for SCF mRNA. In addition, two lines (LS174T and HT29) synthesized SCF protein, as detected by Western blot analysis. SCF stimulated proliferation and colony formation of LS174T in a dose-dependent manner up to 160%. A half-maximal effect was obtained with about 5.5 ng/ml of SCF under both growth conditions. LS174T cells expressed the M(r) 145,000 c-kit protein on the cell surface and a neutralizing anti-c-kit mAb inhibited colony formation of LS174T by 40%. Interleukin 4 (IL-4) completely inhibited SCF-induced proliferation of LS174T cells. Interestingly, IL-4 induced an almost complete down-regulation of both c-kit and SCF expression in LS174T. Our findings suggest that in LS174T cells, an SCF-mediated autocrine loop is functional and that IL-4 down-regulates the expression of both the receptor and the ligand of this circuit.
Keywords
Base Sequence Cell Division/drug effects/genetics Colorectal Neoplasms/*genetics/pathology Down-Regulation Gene Expression Regulation, Neoplastic/*drug effects Humans Interleukin-4/*pharmacology Molecular Sequence Data *Proto-Oncogenes Stem Cell Factor/*biosynthesis Stimulation, Chemical Tumor Cells, Cultured
Pubmed
Web of science
Create date
28/01/2008 8:56
Last modification date
20/08/2019 14:45
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