Changes of beta-amyloid precursor protein splice patterns in brain cell aggregate cultures.

Details

Serval ID
serval:BIB_86571FD8131F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Changes of beta-amyloid precursor protein splice patterns in brain cell aggregate cultures.
Journal
Journal of Neuroscience Research
Author(s)
Gebicke-Haerter P.J., Appel K., Honegger P., Berger M.
ISSN
0360-4012 (Print)
ISSN-L
0360-4012
Publication state
Published
Issued date
1994
Volume
38
Number
1
Pages
32-40
Language
english
Abstract
The splice pattern of beta-amyloid precursor protein (beta-APP) has been studied in a variety of neuronal and glial cells and in brain cell aggregate cultures by the polymerase chain reaction (PCR). The brain-typical pattern, in which beta-APP695 is the dominant form, has been found only in aggregate cultures but not in any of the other cell types including neuronal cell lines. Selective elimination of glial cells from aggregates resulted in increased quantities of beta-APP695, whereas removal of neurons led to a reduction of beta-APP695 and to an elevation of beta-APP751 and beta-APP770. This shift of splice pattern was not observed in cocultures of the neuronal cell line PC 12 with primary astrocytes combined in a variety of cellular ratios. Blood serum, which is an essential component of these cultures, tested on aggregates, did not reduce the amount of beta-APP695 or have any marked effects on splice patterns generally. From these results it is concluded that investigations on brain-typical splicing of beta-APP require primary neurons. Neuronal cell lines may be no suitable model systems. Splicing events favoring production of beta-APP695 may mark an important, very early step of amyloid formation in the brain.
Keywords
Amyloid beta-Protein Precursor/metabolism, Animals, Base Sequence, Brain/cytology, Brain Chemistry/physiology, Cell Aggregation, Cells, Cultured, Culture Media, Conditioned, Molecular Sequence Data, Neuroglia/metabolism, Neurons/metabolism, PC12 Cells, Polymerase Chain Reaction, RNA/biosynthesis, Rats
Pubmed
Web of science
Create date
24/01/2008 13:11
Last modification date
20/08/2019 14:45
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