Role of TNFR1 and TNFR2 in TNF-induced platelet consumption in mice.

Details

Serval ID
serval:BIB_85A6A7FF9F70
Type
Article: article from journal or magazin.
Collection
Publications
Title
Role of TNFR1 and TNFR2 in TNF-induced platelet consumption in mice.
Journal
Journal of Immunology
Author(s)
Tacchini-Cottier F., Vesin C., Redard M., Buurman W., Piguet P.F.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Publication state
Published
Issued date
1998
Volume
160
Number
12
Pages
6182-6186
Language
english
Abstract
An injection of TNF in mice induced profound thrombocytopenia, due to an increase of platelet consumption, that was evident after 1 h and lasted for 3 days. This process was evident in mice that were genetically deficient in TNFR2 (p75) but not in mice lacking TNFR1 (p55), indicating that the process is mediated by TNFR1-bearing cells. To explore the site of action of TNF, labeled platelets from TNFR1 -/- or +/+ donors were transferred to TNFR1 -/- or +/+ recipients. TNF induced the consumption of platelets from TNFR1 -/- donors when injected into +/+ recipients, while platelets from +/+ donors were not consumed when present in TNFR1 -/- recipients; this finding indicates that TNF acts on the TNFR1 of host cells but does not act on platelets. The expression of TNFRs is consistent with this interpretation, since TNFRs were not detected on platelets by flow cytometry. In megakaryocytes, the expression of TNFR1 was detected by immunohistochemistry. These results indicate that TNF induces platelet consumption by acting not on platelets directly but on the TNFR1 of other cells, presumably increasing the release of factors with agonist activity for platelets.
Keywords
Animals, Antigens, CD/physiology, Blood Platelets/drug effects, Humans, Immunohistochemistry, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Knockout, Receptors, Tumor Necrosis Factor/physiology, Receptors, Tumor Necrosis Factor, Type I, Receptors, Tumor Necrosis Factor, Type II, Thrombocytopenia/chemically induced, Thrombocytopenia/immunology, Tumor Necrosis Factor-alpha
Pubmed
Web of science
Create date
24/01/2008 16:09
Last modification date
20/08/2019 15:45
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