Direct vaccination with tumor cells killed with ICP4-deficient HSVd120 elicits effective antitumor immunity.

Details

Serval ID
serval:BIB_8571C4DDA646
Type
Article: article from journal or magazin.
Collection
Publications
Title
Direct vaccination with tumor cells killed with ICP4-deficient HSVd120 elicits effective antitumor immunity.
Journal
Cancer Biology and Therapy
Author(s)
Benencia F., Courrèges M.C., Conejo-García J.R., Mohammed-Hadley A., Coukos G.
ISSN
1538-4047 (Print)
ISSN-L
1538-4047
Publication state
Published
Issued date
2006
Volume
5
Number
7
Pages
867-874
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
We tested whether tumor cells were killed by replication-incompetent recombinant herpes simplex virus (HSV) d120 lacking immediate early gene ICP4 and whether HSVd120-killed tumor cells could be used directly for tumor vaccination. Vaccine efficacy was tested in TC-1, a murine adenocarcinoma transformed with HPV16 E6 and E7, and ID8-Vegf, a murine epithelial ovarian cancer model. HSVd120 killed tumor cells by apoptosis. Tumor cells infected by HSVd120 were engulfed more avidly by immature DCs and induced DC maturation more efficiently than tumor cells killed by ultraviolet B (UVB) radiation. HSVd120 infection induced stronger upregulation of GRP94 than UVB in cells undergoing apoptosis. Immunization of mice with HSVd120-killed cells elicited stronger antitumor T cell response, including tumor reactive interferon-gamma secreting and cytotoxic T cells, and resulted in significantly stronger delay in tumor growth than immunization with UVB-killed tumor cells. Thus, the use of replication-incompetent HSV strains lacking ICP4 offers possible advantages in the preparation of whole tumor cell antigen for direct tumor vaccination.
Keywords
Adenocarcinoma/drug therapy, Animals, Antigens, Neoplasm/immunology, Antigens, Neoplasm/metabolism, Cancer Vaccines/immunology, Cancer Vaccines/therapeutic use, Dendritic Cells/immunology, Disease Models, Animal, Female, Immediate-Early Proteins/genetics, Membrane Glycoproteins/immunology, Membrane Glycoproteins/metabolism, Mice, Ovarian Neoplasms/drug therapy, Simplexvirus/genetics, T-Lymphocytes/immunology, Up-Regulation, Vaccination/methods, Virus Replication/genetics
Pubmed
Web of science
Create date
14/10/2014 12:43
Last modification date
20/08/2019 15:44
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