Carbon-14-urea breath test as a noninvasive method to monitor Helicobacter felis colonization in mice
Details
Serval ID
serval:BIB_856C5BA4B0A0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Carbon-14-urea breath test as a noninvasive method to monitor Helicobacter felis colonization in mice
Journal
Digestion
ISSN
0012-2823 (Print)
Publication state
Published
Issued date
1996
Volume
57
Number
1
Pages
30-4
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Abstract
We have developed a 14C-urea breath test to follow the course of Helicobacter felis infection in mice. Peak 14CO2 production occurred approximately 8 min after substrate administration. The test values were compared to those from a rapid urease test and correlated with the presence of pathogens by histology. The sensitivity was 99%, specificity 91%, positive predictive value 95% and negative predictive value 99% when the assay was conducted in fasted mice. We conclude that in mice the breath test analysis is a useful noninvasive method for detecting the presence of H. felis or for evaluating therapeutic agents affecting growth or survival of the organism.
Keywords
Animals
Breath Tests/*methods
Carbon Radioisotopes/diagnostic use
Female
Helicobacter/*isolation & purification
Helicobacter Infections/diagnosis/*microbiology/therapy
Mice
Mice, Inbred BALB C
Sensitivity and Specificity
Stomach/*microbiology/pathology
Urea/diagnostic use
Pubmed
Web of science
Create date
25/01/2008 17:02
Last modification date
20/08/2019 15:44