Precisely positioned nucleosomes are not essential for c-fos gene regulation in vivo

Details

Serval ID
serval:BIB_856063B40714
Type
Article: article from journal or magazin.
Collection
Publications
Title
Precisely positioned nucleosomes are not essential for c-fos gene regulation in vivo
Journal
Gene
Author(s)
Fivaz  J., Bassi  M. C., Price  M., Pinaud  S., Mirkovitch  J.
ISSN
0378-1119 (Print)
Publication state
Published
Issued date
09/2000
Volume
255
Number
2
Pages
169-84
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 19
Abstract
Chromatin architecture plays a decisive role in many aspects of transcription regulation. We have tested the role of specific chromatin structures in c-fos gene regulation, using a gene transfer system based on episomes derived from the Epstein-Barr virus (EBV). This system reproduces in several respects the chromatin structure and regulation of the chromosomal c-fos gene. Using this approach, we first demonstrate that the pausing of RNA polymerase II downstream of the transcriptional start site does not require precisely positioned nucleosomes. Indeed, changing the pattern of MNase hypersensitive sites along the transcribed sequence does not perturb RNA polymerase II pausing or the regulation of the c-fos gene. Next, we show that a putative nucleosome positioned between the SIE/SRE elements (-300) and the CRE/TATA elements (-36) is not necessary for activation by a variety of inducers. Accordingly, total or partial deletion of the putative nucleosome sequence does not disturb c-fos regulation while the two regulatory sites flanking the nucleosome sequence remain hypersensitive to MNase. As described in this paper, EBV episomes are useful vectors to critically examine the role of the chromatin structure in gene transcription for human cells.
Keywords
Animals Cell Line Chromatin/genetics DNA/genetics/metabolism Dose-Response Relationship, Drug Forskolin/pharmacology Gene Expression Regulation/drug effects Herpesvirus 4, Human/genetics Humans Ionomycin/pharmacology Jurkat Cells Luciferases/genetics/metabolism Mice Nucleosomes/*genetics Plasmids/genetics Promoter Regions (Genetics)/genetics Proto-Oncogene Proteins c-fos/*genetics RNA Polymerase II/metabolism Recombinant Fusion Proteins/drug effects/genetics/metabolism Tetradecanoylphorbol Acetate/pharmacology Tumor Cells, Cultured
Pubmed
Web of science
Create date
25/01/2008 8:41
Last modification date
20/08/2019 14:44
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