Evidence for heterogeneous TCR V beta repertoire expression in mercury-induced immune disorders in rats.

Details

Serval ID
serval:BIB_851
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Evidence for heterogeneous TCR V beta repertoire expression in mercury-induced immune disorders in rats.
Journal
International Immunology
Author(s)
Fillion J., Baccala R., Pannetier C., Kuhn J., Druet P., Bellon B.
ISSN
0953-8178
Publication state
Published
Issued date
1997
Volume
9
Number
2
Pages
263-271
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
Administration of subtoxic doses of HgCl2 affects differentially the immune system depending on the strain of rats tested. Susceptible Brown-Norway (BN) rats exhibit a CD4+ T cell-dependent polyclonal activation of B cells; in contrast, Lewis (LEW) rats are resistant and develop an immunosuppression mediated by CD8+ T cells recruited by CD4+ T cells. The mechanisms by which mercury induces immune disorders are poorly understood. We were interested in analyzing the diversity and mercury-mediated changes of the TCR Vbeta repertoire in the BN and LEW strains of rats at different times of HgCl2 exposure. Our results obtained after analysis of lymph node T cells by RNase protection assay, flow cytometry or immunoscope assay (i) were not consistent with a superantigen-like stimulus since we observed neither a V beta-selective expansion nor deletion that would have been expected and (ii) showed that in BN rats, as well as in LEW rats, an increase in the number of T cells was associated with the heterogeneous TCR V beta repertoire, thus supporting a polyclonal T cell activation. However, in BN rats the total number of T cells increased very rapidly, whereas in LEW rats only CD8+ T cells accumulated.
Keywords
Animals, Antigens, CD4/chemistry, Antigens, CD8/chemistry, Autoimmune Diseases/chemically induced, Autoimmune Diseases/immunology, Female, Flow Cytometry, Immunoglobulin Variable Region/classification, Lymph Nodes/cytology, Lymph Nodes/drug effects, Lymphocyte Count/drug effects, Male, Mercury/adverse effects, Mercury/immunology, Rats, Rats, Inbred BN, Rats, Inbred Lew, Receptors, Antigen, T-Cell, alpha-beta/biosynthesis, Receptors, Antigen, T-Cell, alpha-beta/classification, T-Lymphocyte Subsets/cytology, T-Lymphocyte Subsets/drug effects
Pubmed
Web of science
Create date
19/11/2007 13:46
Last modification date
20/08/2019 15:44
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