Synthesis of polyhydroxyalkanoate in the peroxisome of Pichia pastoris.

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Serval ID
serval:BIB_84E48D5D6DC2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Synthesis of polyhydroxyalkanoate in the peroxisome of Pichia pastoris.
Journal
FEMS Microbiology Letters
Author(s)
Poirier Y., Erard N., MacDonald-Comber Petétot J.
ISSN
0378-1097 (Print)
ISSN-L
0378-1097
Publication state
Published
Issued date
2002
Volume
207
Number
1
Pages
97-102
Language
english
Abstract
Polyhydroxyalkanoates (PHAs) are polyesters naturally produced by bacteria that have properties of biodegradable plastics and elastomers. A PHA synthase from Pseudomonas aeruginosa modified at the carboxy-end for peroxisomal targeting was transformed in Pichia pastoris. The PHA synthase was expressed under the control of the promoter of the P. pastoris acyl-CoA oxidase gene. Synthesis of up to 1% medium-chain-length PHA per g dry weight was dependent on both the expression of the PHA synthase and the presence of oleic acid in the medium. PHA accumulated as inclusions within the peroxisomes. P. pastoris could be used as a model system to study how peroxisomal metabolism needs to be modified to increase PHA production in other eukaryotes, such as plants.
Keywords
Acyltransferases/genetics, Acyltransferases/metabolism, Culture Media, Microscopy, Electron, Peroxisomes/metabolism, Peroxisomes/ultrastructure, Pichia/enzymology, Pichia/genetics, Polyesters/metabolism, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics, Recombination, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 19:42
Last modification date
25/09/2019 6:09
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