T cell response to purified filtrate antigen 85 from Mycobacterium bovis Bacilli Calmette-Guérin (BCG) in leprosy patients.

Details

Serval ID
serval:BIB_84E05FA0494E
Type
Article: article from journal or magazin.
Collection
Publications
Title
T cell response to purified filtrate antigen 85 from Mycobacterium bovis Bacilli Calmette-Guérin (BCG) in leprosy patients.
Journal
Clinical and Experimental Immunology
Author(s)
Launois P., Huygen K., De Bruyn J., N'Diaye M., Diouf B., Sarthouj L., Grimaud J., Millan J.
ISSN
0009-9104 (Print)
ISSN-L
0009-9104
Publication state
Published
Issued date
1991
Volume
86
Number
2
Pages
286-290
Language
english
Abstract
T cell proliferation and IFN-gamma production of peripheral blood mononuclear cells from 25 healthy controls and 39 leprosy patients were tested against BCG-bacilli and culture filtrate. Mycobacterium leprae and purified antigen 85 (the major secreted 30-32 kD protein antigen) from M. bovis strain BCG. In lepromin negative healthy controls, blastogenesis was low to M. leprae and completely negative to antigen 85. IFN-gamma levels were very low, close to detection limits. In all lepromin positive controls, significant proliferation and IFN-gamma secretion was found in response to M. leprae and antigen 85. In the group of lepromatous leprosy (LL) patients, 25/29 of patients (with either positive (13) or negative (12) lymphoproliferative response to BCG) were unreactive to M. leprae or to antigen 85. Four LL patients with positive T cell response to BCG responded with detectable lymphoproliferative response and IFN-gamma secretion to antigen 85. All tuberculoid (TT) leprosy patients responded to BCG, M. leprae and antigen 85. Hence, T cells from leprosy patients and controls demonstrate a marked parallelism of responsiveness towards whole M. leprae and purified antigen 85 from M. bovis BCG, suggesting strong cross-reactivity between the two species and underlining the biological importance of such secreted antigens.
Keywords
Antigens, Bacterial/immunology, Bacterial Proteins/immunology, Humans, Interferon-gamma/biosynthesis, Leprosy, Lepromatous/immunology, Leprosy, Tuberculoid/immunology, Lymphocyte Activation, Mycobacterium bovis/immunology, Mycobacterium leprae/immunology, T-Lymphocytes/immunology
Pubmed
Web of science
Create date
28/01/2008 11:05
Last modification date
20/08/2019 14:44
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