Cellular gene expression survey of vaccinia virus infection of human HeLa cells.

Details

Serval ID
serval:BIB_84CB97B07938
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cellular gene expression survey of vaccinia virus infection of human HeLa cells.
Journal
Journal of Virology
Author(s)
Guerra S., Lopez-Fernandez L.A., Pascual-Montano A., Munoz M., Harshman K., Esteban M.
ISSN
0022-538X[print], 0022-538X[linking]
Publication state
Published
Issued date
2003
Volume
77
Number
11
Pages
6493-6506
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Vaccinia virus (VV) is a cytocidal virus that causes major changes in host cell machinery shortly after infecting cells. To define the consequences of virus infection on host gene expression, we used microarrays of approximately 15,000 human cDNAs to examine expression levels of mRNAs isolated at 2, 6, and 16 h postinfection from cultures of infected HeLa cells. The majority of profiling changes during VV infection corresponded to downregulation of genes at 16 h postinfection. Differentially expressed genes were clustered into seven groups to identify common regulatory pathways, with most of them (90%) belonging to clusters 6 and 7, which represent genes whose expression was repressed after infection. Cluster 1, however, contained 37 transcripts (2.81%) showing a robust pattern of induction that was maintained during the course of infection. Genes in cluster 1 included those for Wiskott-Aldrich syndrome protein (WASP) family member WASF1, thymosine, adenosine A2a receptor, glutamate decarboxylase 2, CD-80 antigen, KIAA0888 protein, selenophosphate synthetase, pericentrin, and attractin as well as several expressed sequence tags. We analyzed in more detail the fate of WASP protein in VV-infected cells, because a related family member, N-WASP, is involved in viral motility. WASP protein accumulated in the course of infection; its increase required viral DNA replication and de novo protein synthesis, and it localized in cytoplasmic structures distinct from uninfected cells. This study is the first quantitative analysis of host gene expression following VV infection of cultured human cells, demonstrating global changes in the expression profile, and identifies upregulated genes with potential roles in the virus replication cycle.
Keywords
Blotting, Western, Fluorescent Antibody Technique, Gene Expression Profiling, Gene Expression Regulation, Hela Cells/virology, Humans, Oligonucleotide Array Sequence Analysis, Proteins/genetics, Proteins/metabolism, RNA, Messenger/genetics, RNA, Messenger/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Vaccinia virus/pathogenicity, Vaccinia virus/physiology, Virus Replication
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:33
Last modification date
20/08/2019 14:44
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