Octapeptide-specific and sensitive assay for angiotensin II in plasma

Details

Serval ID
serval:BIB_8481DC02E370
Type
Article: article from journal or magazin.
Collection
Publications
Title
Octapeptide-specific and sensitive assay for angiotensin II in plasma
Journal
International Journal of Environmental Analytical Chemistry
Author(s)
Nussberger  J., Brunner  D. B., Waeber  B., Brunner  H. R.
ISSN
0306-7319 (Print)
Publication state
Published
Issued date
1986
Volume
25
Number
1-3
Pages
257-68
Notes
Journal Article
Research Support, Non-U.S. Gov't
Abstract
Angiotensin-(1-8)octapeptide (angiotensin II) is the active principle of the renin-angiotensin system. Crossreaction of angiotensin II-antisera with inactive precursors and metabolic fragments prevented the specific quantitation of this hormone in biological fluids. Peptide-extraction on bonded-phase silica followed by peptide-separation using isocratic reverse-phase high performance liquid chromatography and subsequent radioimmunoassay rendered possible the octapeptide-specific measurement of angiotensin II in 2 ml plasma with a detection limit of 0.4 fmol/ml. The coefficient of variation for intra-assay precision was 0.06 and for inter-assay precision 0.13. 125I-angiotensin II was recovered from plasma by solid-phase extraction to 99 +/- 2% (mean +/- S.D.). The overall recovery of 5, 10 and 20 fmol unlabeled angiotensin II added to plasma was 80 +/- 10%. Plasma concentrations in supine normal humans averaged 4.1 +/- 1.6 fmol/ml and were suppressed below the detection limit by angiotensin I converting enzyme inhibition.
Keywords
Angiotensin II/*blood Chromatography, High Pressure Liquid Cross Reactions Humans Indicators and Reagents Radioimmunoassay
Pubmed
Web of science
Create date
05/03/2008 16:39
Last modification date
20/08/2019 14:44
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