Limiting factors in single particle cryo electron tomography.

Details

Serval ID
serval:BIB_83EAF68E5CE4
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Limiting factors in single particle cryo electron tomography.
Journal
Computational and structural biotechnology journal
Author(s)
Kudryashev M., Castaño-Díez D., Stahlberg H.
ISSN
2001-0370 (Print)
ISSN-L
2001-0370
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
1
Pages
e201207002
Language
english
Notes
Publication types: Journal Article ; Review
Publication Status: epublish
Abstract
Modern methods of cryo electron microscopy and tomography allow visualization of protein nanomachines in their native state at the nanometer scale. Image processing methods including sub-volume averaging applied to repeating macromolecular elements within tomograms allow exploring their structures within the native context of the cell, avoiding the need for protein isolation and purification. Today, many different data acquisition protocols and software solutions are available to researchers to determine average structures of macromolecular complexes and potentially to classify structural intermediates. Here, we list the density maps reported in the literature, and analyze each structure for the chosen instrumental settings, sample conditions, main processing steps, and obtained resolution. We present conclusions that identify factors currently limiting the resolution gained by this approach.
Pubmed
Open Access
Yes
Create date
09/06/2023 16:03
Last modification date
28/07/2023 6:59
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