Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_83998924219B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Memo1 gene expression in kidney and bone is unaffected by dietary mineral load and calciotropic hormones.
Journal
Physiological reports
Author(s)
Moor M.B., Bonny O.
ISSN
2051-817X (Electronic)
ISSN-L
2051-817X
Publication state
Published
Issued date
04/2020
Peer-reviewed
Oui
Volume
8
Number
7
Pages
e14410
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Mediator of cell motility 1 (MEMO1) is a ubiquitously expressed modulator of cellular responses to growth factors including FGF23 signaling, and Memo1-deficient mice share some phenotypic traits with Fgf23- or Klotho-deficient mouse models. Here, we tested whether Memo1 gene expression is regulated by calciotropic hormones or by changing the dietary mineral load. MLO-Y4 osteocyte-like cells were cultured and treated with 1,25(OH) <sub>2</sub> -vitamin D <sub>3</sub> . Wild-type C57BL/6N mice underwent treatments with 1,25(OH) <sub>2</sub> -vitamin D <sub>3</sub> , parathyroid hormone, 17β-estradiol or vehicle. Other cohorts of C57BL/6N mice were fed diets varying in calcium or phosphate content. Expression of Memo1 and control genes was assessed by qPCR. 1,25(OH) <sub>2</sub> -vitamin D <sub>3</sub> caused an acute decrease in Memo1 transcript levels in vitro, but not in vivo. None of the hormones tested had an influence on Memo1 transcripts, whereas the assessed control genes reacted the expected way. Dietary interventions with calcium and phosphate did not affect Memo1 transcripts but altered the chosen control genes' expression. We observed that Memo1 was not regulated by calciotropic hormones or change in mineral load, suggesting major differences between the regulation and physiological roles of Klotho, Fgf23, and Memo1.
Keywords
FGF23, Memo1, calcium, phosphate, vitamin D3
Pubmed
Open Access
Yes
Create date
25/04/2020 20:43
Last modification date
03/04/2021 6:31
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