New genome assembly of the barn owl (Tyto alba alba).

Details

Serval ID
serval:BIB_83891497D9E8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
New genome assembly of the barn owl (Tyto alba alba).
Journal
Ecology and evolution
Author(s)
Ducrest A.L., Neuenschwander S., Schmid-Siegert E., Pagni M., Train C., Dylus D., Nevers Y., Warwick Vesztrocy A., San-Jose L.M., Dupasquier M., Dessimoz C., Xenarios I., Roulin A., Goudet J.
ISSN
2045-7758 (Print)
ISSN-L
2045-7758
Publication state
Published
Issued date
03/2020
Peer-reviewed
Oui
Volume
10
Number
5
Pages
2284-2298
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
New genomic tools open doors to study ecology, evolution, and population genomics of wild animals. For the Barn owl species complex, a cosmopolitan nocturnal raptor, a very fragmented draft genome was assembled for the American species (Tyto furcata pratincola) (Jarvis et al. 2014). To improve the genome, we assembled de novo Illumina and Pacific Biosciences (PacBio) long reads sequences of its European counterpart (Tyto alba alba). This genome assembly of 1.219 Gbp comprises 21,509 scaffolds and results in a N50 of 4,615,526 bp. BUSCO (Universal Single-Copy Orthologs) analysis revealed an assembly completeness of 94.8% with only 1.8% of the genes missing out of 4,915 avian orthologs searched, a proportion similar to that found in the genomes of the zebra finch (Taeniopygia guttata) or the collared flycatcher (Ficedula albicollis). By mapping the reads of the female American barn owl to the male European barn owl reads, we detected several structural variants and identified 70 Mbp of the Z chromosome. The barn owl scaffolds were further mapped to the chromosomes of the zebra finch. In addition, the completeness of the European barn owl genome is demonstrated with 94 of 128 proteins missing in the chicken genome retrieved in the European barn owl transcripts. This improved genome will help future barn owl population genomic investigations.
Keywords
Strigiformes, Tytonidae, assembly, barn owl, bird, genome
Pubmed
Web of science
Open Access
Yes
Create date
01/04/2020 21:36
Last modification date
16/04/2020 6:26
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