Virus BK en injertos renales: evidencia histológica de la infección [BK virus in kidney allografts: a search for histologic evidence of the infection]
Details
Serval ID
serval:BIB_832FBCAF1FCA
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Institution
Title
Virus BK en injertos renales: evidencia histológica de la infección [BK virus in kidney allografts: a search for histologic evidence of the infection]
Journal
Nefrologia
ISSN
0211-6995 (Print)
ISSN-L
0211-6995
Publication state
Published
Issued date
2004
Peer-reviewed
Oui
Volume
24
Number
5
Pages
480-485
Language
spanish
Notes
Publication types: Case Reports ; English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
BK virus (BKV) associated interstitial nephritis is a complication in renal transplantation recipients. Its incidence is controversial. The aim of the present study is to determine the incidence of histopathologic evidence of BKV-infection in a single centre.
Renal allograft tissue samples (n = 838) from 526 patients undergoing renal transplant were evaluated by light microscopy. Polymerase chain reaction (PCR) assay for BKV DNA was performed in 41 microdissected cell populations from cases with viral inclusions, cases with other nuclear changes, and cases without nuclear changes.
Polyomavirus-inclusions were identified in six cases (five with interstitial nephritis and other one with only urothelial infection). In one case with interstitial nephritis the DNA was degraded. PCR confirmed BKV infection in the other five and was negative in cases without inclusions.
Five patterns of inclusions bodies are observed and they appear to be characteristic. PCR assay seemingly have a high specificity for BKV detection and it does not usually detect latent viral infection.
Renal allograft tissue samples (n = 838) from 526 patients undergoing renal transplant were evaluated by light microscopy. Polymerase chain reaction (PCR) assay for BKV DNA was performed in 41 microdissected cell populations from cases with viral inclusions, cases with other nuclear changes, and cases without nuclear changes.
Polyomavirus-inclusions were identified in six cases (five with interstitial nephritis and other one with only urothelial infection). In one case with interstitial nephritis the DNA was degraded. PCR confirmed BKV infection in the other five and was negative in cases without inclusions.
Five patterns of inclusions bodies are observed and they appear to be characteristic. PCR assay seemingly have a high specificity for BKV detection and it does not usually detect latent viral infection.
Keywords
Adult, BK Virus/genetics, BK Virus/isolation & purification, DNA, Viral/analysis, Female, Humans, Incidence, Kidney/pathology, Kidney/virology, Kidney Transplantation/pathology, Male, Middle Aged, Nephritis, Interstitial/epidemiology, Nephritis, Interstitial/pathology, Nephritis, Interstitial/virology, Polymerase Chain Reaction, Polyomavirus Infections/epidemiology, Polyomavirus Infections/pathology, Polyomavirus Infections/virology, Transplants/virology, Tumor Virus Infections/epidemiology, Tumor Virus Infections/pathology, Tumor Virus Infections/virology
Pubmed
Web of science
Create date
13/02/2018 10:32
Last modification date
20/08/2019 15:43
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