High mobility group chromosomal protein 1 binds to the adeno-associated virus replication protein (Rep) and promotes Rep-mediated site-specific cleavage of DNA, ATPase activity and transcriptional repression.

Details

Serval ID
serval:BIB_832
Type
Article: article from journal or magazin.
Collection
Publications
Title
High mobility group chromosomal protein 1 binds to the adeno-associated virus replication protein (Rep) and promotes Rep-mediated site-specific cleavage of DNA, ATPase activity and transcriptional repression.
Journal
EMBO Journal
Author(s)
Costello E., Saudan P., Winocour E., Pizer L., Beard P.
ISSN
0261-4189
Publication state
Published
Issued date
1997
Volume
16
Number
19
Pages
5943-5954
Language
english
Notes
Publication types: Journal Article
Abstract
High mobility group protein 1 (HMG1) is an abundant non-histone chromosomal protein which plays a role in several nuclear events involving DNA. Here we demonstrate that HMG1 physically interacts with the human adeno-associated virus (AAV) Rep protein. HMG1 promotes the formation of Rep-DNA complexes and stimulates the activity of Rep in site- and strand-specific cleavage of DNA and the hydrolysis of ATP, functions required for viral gene regulation, replication and site-specific integration of viral DNA into human chromosome 19. We show that HMG1 enhances Rep-mediated repression of the AAV p5 promoter in transfected cells, suggesting that HMG1 and Rep also interact in vivo. HMG1, Rep and DNA can be immunoprecipitated as a ternary complex. Kinetic studies indicate that complexes of Rep with DNA have similar stabilities in the presence and absence of HMG1.These results suggest that the effect of HMG1 on Rep binding is exerted at the step of complex formation and thereby may reflect an activity of HMG1 in promoting the assembly of complex cellular nucleoprotein structures.
Keywords
Adenosine Triphosphatases/metabolism, Binding Sites, DNA/metabolism, DNA-Binding Proteins/metabolism, Dependovirus, Gene Expression Regulation, Viral, Hela Cells, High Mobility Group Proteins/metabolism, Humans, Protein Binding, Repressor Proteins/metabolism, Transcription, Genetic, Viral Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 13:46
Last modification date
08/05/2019 21:15
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