Epstein-Barr virus burden in Hodgkin's disease is related to latent membrane protein gene expression but not to active viral replication.

Details

Serval ID
serval:BIB_82EDF9AE9D27
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Epstein-Barr virus burden in Hodgkin's disease is related to latent membrane protein gene expression but not to active viral replication.
Journal
Blood
Author(s)
Joske D.J., Emery-Goodman A., Bachmann E., Bachmann F., Odermatt B., Knecht H.
ISSN
0006-4971 (Print)
ISSN-L
0006-4971
Publication state
Published
Issued date
15/11/1992
Peer-reviewed
Oui
Volume
80
Number
10
Pages
2610-2613
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The Epstein-Barr virus (EBV) has been increasingly detected in Hodgkin's disease (HD), but its role in pathogenesis remains uncertain. We analyzed 20 specimens of HD known to contain EBV DNA by a sensitive reverse transcriptase polymerase chain reaction (RT-PCR). The cases were assessed for the presence of RNA transcripts of the BNLF1 gene (coding for the viral latent membrane protein [LMP]) and the late replicative gene BLLF1 (coding for the principle envelope glycoprotein [gp220/350]). LMP RNA transcripts were found in 9 of 20 (45%) cases, mostly those containing many copies of viral DNA and of mixed cellularity (MC) histological subtype. Only one LMP RNA-positive case was also positive for RNA transcripts of the active replication gene BLLF1. Our results show that viral burden in HD is not primarily related to active viral replication, but is associated with LMP gene expression.
Keywords
Adolescent, Adult, Aged, Aged, 80 and over, Antigens, Viral/genetics, Base Sequence, Child, DNA, Viral/analysis, Female, Gene Expression, Herpesvirus 4, Human/genetics, Hodgkin Disease/microbiology, Humans, Male, Membrane Proteins/genetics, Middle Aged, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Viral/analysis, RNA-Directed DNA Polymerase, Transcription, Genetic, Viral Matrix Proteins, Virus Replication/genetics
Pubmed
Web of science
Create date
18/05/2014 16:42
Last modification date
03/02/2024 8:13
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