FRAP Assay to Trace Lipid Mixing of the Inner and Outer Leaflet of Yeast Vacuoles: Assessing the Fusion State in Live Cells
Details
Serval ID
serval:BIB_82DE33222C35
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
FRAP Assay to Trace Lipid Mixing of the Inner and Outer Leaflet of Yeast Vacuoles: Assessing the Fusion State in Live Cells
Title of the book
Methods in Molecular Biology
Publisher
Springer
ISBN
9781071643136
9781071643143
9781071643143
ISSN
1064-3745
1940-6029
1940-6029
ISSN-L
1064-3745
Publication state
Published
Issued date
2025
Peer-reviewed
Oui
Volume
2887
Chapter
14
Pages
197-206
Language
english
Abstract
Fluorescence recovery after photobleaching (FRAP) can be employed to investigate membrane lipid mixing of vacuoles in live budding yeast cells and distinguish the fused, hemi-fused or non-fused states of these organelles under physiological conditions. Here, we describe a protocol for labeling the outer and inner leaflets of vacuoles in live cells that allow to detect hemifusion intermediates and, thus, identify components necessary for fusion pore opening.
Pubmed
Create date
17/01/2025 13:28
Last modification date
18/01/2025 7:08