Buccal swabs: a universal alternative to sample bird DNA

Details

Serval ID
serval:BIB_82322FE13851
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Buccal swabs: a universal alternative to sample bird DNA
Journal
Bulletin de la Société Vaudoise des Sciences Naturelles
Author(s)
Lavanchy Guillaume, Dufresnes Christophe
ISSN
0037-9603
Publication state
Published
Issued date
2020
Peer-reviewed
Oui
Volume
99
Pages
73-81
Language
english
Abstract
Least intrusive sampling should be prioritized for wildlife conservation and bioethics. In birds, DNA samples are typically obtained from blood. Here we emphasized buccal swabs as an alternative source. We tested swabs in adults and nestlings representative of 17 European species (n 189), ranging from small passerines to large Strigiformes (owls) and Larids (gulls), and characterized the extracted DNA yields. The contents were highly variable between and within species, both in terms of quantity and perhaps purity, but the amounts obtained (-100— 10,000 ng) meet the requirements of standard molecular analyses. No differences were found between adults and nestlings. The DNA yields were moderately higher for larger bird species, and samples taken from experienced swabbers yielded more DNA. In line with species-specific case studies that had successfully implemented buccal swabs, our results support the universal potential of the technique, which should be more generally applied in avian research, notably when endangered species are involved.
Create date
04/01/2022 10:13
Last modification date
05/01/2022 6:37
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