Characterization of hepatic fatty acids in mice with reduced liver fat by ultra-short echo time (1)H-MRS at 14.1 T in vivo.

Details

Serval ID
serval:BIB_81B801E31A4E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization of hepatic fatty acids in mice with reduced liver fat by ultra-short echo time (1)H-MRS at 14.1 T in vivo.
Journal
Nmr In Biomedicine
Author(s)
Soares A.F., Lei H., Gruetter R.
ISSN
1099-1492 (Electronic)
ISSN-L
0952-3480
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
28
Number
8
Pages
1009-1020
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Alterations in the hepatic lipid content (HLC) and fatty acid composition are associated with disruptions in whole body metabolism, both in humans and in rodent models, and can be non-invasively assessed by (1)H-MRS in vivo. We used (1)H-MRS to characterize the hepatic fatty-acyl chains of healthy mice and to follow changes caused by streptozotocin (STZ) injection. Using STEAM at 14.1 T with an ultra-short TE of 2.8 ms, confounding effects from T2 relaxation and J-coupling were avoided, allowing for accurate estimations of the contribution of unsaturated (UFA), saturated (SFA), mono-unsaturated (MUFA) and poly-unsaturated (PUFA) fatty-acyl chains, number of double bonds, PU bonds and mean chain length. Compared with in vivo (1) H-MRS, high resolution NMR performed in vitro in hepatic lipid extracts reported longer fatty-acyl chains (18 versus 15 carbons) with a lower contribution from UFA (61 ± 1% versus 80 ± 5%) but a higher number of PU bonds per UFA (1.39 ± 0.03 versus 0.58 ± 0.08), driven by the presence of membrane species in the extracts. STZ injection caused a decrease of HLC (from 1.7 ± 0.3% to 0.7 ± 0.1%), an increase in the contribution of SFA (from 21 ± 2% to 45 ± 6%) and a reduction of the mean length (from 15 to 13 carbons) of cytosolic fatty-acyl chains. In addition, SFAs were also likely to have increased in membrane lipids of STZ-induced diabetic mice, along with a decrease of the mean chain length. These studies show the applicability of (1)H-MRS in vivo to monitor changes in the composition of the hepatic fatty-acyl chains in mice even when they exhibit reduced HLC, pointing to the value of this methodology to evaluate lipid-lowering interventions in the scope of metabolic disorders.
Keywords
Adipose Tissue/metabolism, Adiposity, Animals, Biomarkers/metabolism, Diabetes Mellitus, Experimental/chemically induced, Diabetes Mellitus, Experimental/metabolism, Fatty Acids/metabolism, Liver/metabolism, Mice, Mice, Inbred C57BL, Proton Magnetic Resonance Spectroscopy/methods, Reproducibility of Results, Sensitivity and Specificity, Streptozocin, Tissue Distribution
Pubmed
Web of science
Create date
24/08/2015 16:26
Last modification date
20/08/2019 14:42
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