Distinct nitrogen isotopic compositions of healthy and cancerous tissue in mice brain and head&neck micro-biopsies.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_81B033323C18
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Distinct nitrogen isotopic compositions of healthy and cancerous tissue in mice brain and head&neck micro-biopsies.
Journal
BMC cancer
Author(s)
Straub M., Sigman D.M., Auderset A., Ollivier J., Petit B., Hinnenberg B., Rubach F., Oleynik S., Vozenin M.C., Martínez-García A.
ISSN
1471-2407 (Electronic)
ISSN-L
1471-2407
Publication state
Published
Issued date
13/07/2021
Peer-reviewed
Oui
Volume
21
Number
1
Pages
805
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Cancerous cells can recycle metabolic ammonium for their growth. As this ammonium has a low nitrogen isotope ratio ( <sup>15</sup> N/ <sup>14</sup> N), its recycling may cause cancer tissue to have lower <sup>15</sup> N/ <sup>14</sup> N than surrounding healthy tissue. We investigated whether, within a given tissue type in individual mice, tumoral and healthy tissues could be distinguished based on their <sup>15</sup> N/ <sup>14</sup> N.
Micro-biopsies of murine tumors and adjacent tissues were analyzed for <sup>15</sup> N/ <sup>14</sup> N using novel high-sensitivity methods. Isotopic analysis was pursued in Nude and C57BL/6 mice models with mature orthotopic brain and head&neck tumors generated by implantation of H454 and MEERL95 murine cells, respectively.
In the 7 mice analyzed, the brain tumors had distinctly lower <sup>15</sup> N/ <sup>14</sup> N than healthy neural tissue. In the 5 mice with head&neck tumors, the difference was smaller and more variable. This was at least partly due to infiltration of healthy head&neck tissue by tumor cells. However, it may also indicate that the <sup>15</sup> N/ <sup>14</sup> N difference between tumoral and healthy tissue depends on the nitrogen metabolism of the healthy organ in question.
The findings, coupled with the high sensitivity of the <sup>15</sup> N/ <sup>14</sup> N measurement method used here, suggest a new approach for micro-biopsy-based diagnosis of malignancy as well as an avenue for investigation of cancer metabolism.
Keywords
Cell metabolism, Diagnostics, Micro-biopsies, Nitrogen isotopes
Pubmed
Web of science
Open Access
Yes
Create date
15/07/2021 14:57
Last modification date
12/01/2022 8:11
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