Recognition of peptide orientation: studies with angiotensin II in the guinea-pig
Details
Serval ID
serval:BIB_8178C79DE58F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Recognition of peptide orientation: studies with angiotensin II in the guinea-pig
Journal
Molecular Immunology
ISSN
0161-5890 (Print)
Publication state
Published
Issued date
06/1985
Volume
22
Number
6
Pages
619-21
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
Antipeptide sera of defined subspecificities were obtained when angiotensin II was unidirectionally conjugated to its carrier protein. Antisera with predominantly amino terminal specificity were produced when guinea-pigs were immunized with angiotensin II conjugated via its carboxy terminus to thyroglobulin. When angiotensin II was coupled via its amino terminus, carboxy terminal-specific antisera were obtained. The same conjugates failed to provide antisera with the corresponding specificities when rabbits were used instead of guinea-pigs. This work demonstrates that the peptide-protein coupling strategy may affect the specificity of the resulting antiserum.
Keywords
Angiotensin II/*immunology
Animals
*Antibody Specificity
Cross Reactions
Epitopes/*immunology
Female
Guinea Pigs
Immune Sera/immunology
Thyroglobulin/immunology
Pubmed
Web of science
Create date
05/03/2008 17:40
Last modification date
20/08/2019 15:41