EML1 is essential for retinal photoreceptor migration and survival.

Details

Serval ID
serval:BIB_80FFE0A767CA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
EML1 is essential for retinal photoreceptor migration and survival.
Journal
Scientific reports
Author(s)
Poria D., Sun C., Santeford A., Kielar M., Apte R.S., Kisselev O.G., Chen S., Kefalov V.J.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
21/02/2022
Peer-reviewed
Oui
Volume
12
Number
1
Pages
2897
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Calcium regulates the response sensitivity, kinetics and adaptation in photoreceptors. In striped bass cones, this calcium feedback includes direct modulation of the transduction cyclic nucleotide-gated (CNG) channels by the calcium-binding protein CNG-modulin. However, the possible role of EML1, the mammalian homolog of CNG-modulin, in modulating phototransduction in mammalian photoreceptors has not been examined. Here, we used mice expressing mutant Eml1 to investigate its role in the development and function of mouse photoreceptors using immunostaining, in-vivo and ex-vivo retinal recordings, and single-cell suction recordings. We found that the mutation of Eml1 causes significant changes in the mouse retinal structure characterized by mislocalization of rods and cones in the inner retina. Consistent with the fraction of mislocalized photoreceptors, rod and cone-driven retina responses were reduced in the mutants. However, the Eml1 mutation had no effect on the dark-adapted responses of rods in the outer nuclear layer. Notably, we observed no changes in the cone sensitivity in the Eml1 mutant animals, either in darkness or during light adaptation, ruling out a role for EML1 in modulating cone CNG channels. Together, our results suggest that EML1 plays an important role in retina development but does not modulate phototransduction in mammalian rods and cones.
Keywords
Animals, Calcium/physiology, Cell Movement/genetics, Cell Survival/genetics, Cyclic Nucleotide-Gated Cation Channels/metabolism, Mice, Microtubule-Associated Proteins/genetics, Microtubule-Associated Proteins/metabolism, Microtubule-Associated Proteins/physiology, Mutation, Retina/pathology, Retinal Cone Photoreceptor Cells/pathology, Retinal Cone Photoreceptor Cells/physiology, Retinal Rod Photoreceptor Cells/pathology, Retinal Rod Photoreceptor Cells/physiology, Vision, Ocular/genetics
Pubmed
Web of science
Open Access
Yes
Create date
28/02/2022 10:48
Last modification date
23/08/2022 6:45
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